中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode 园艺学报(英文版) 2023,9 (1)

Position: Home > Articles > Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode Horticultural Plant Journal 2023,9 (1)

Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode

作者：

Yang Liu;Hongyi Cao;Jian Ling;Yuhong Yang;Yan Li;Bingyan Xie;Jianlong Zhao;Zhenchuan Ma

关键词：

Capsicum annuum L.;Meloidogyne incognita;Nematode-resistant gene;Fine mapping;Transgenic technolog

摘要：

Root-knot nematodes (RKNs) cause severe diseases in peppers annually around the world. In pepper, the Me3 gene provides a heat-stable and broad-spectrum resistance to RKNs. In this study, several simple sequence repeat (SSR) markers and insertion/deletion (InDel) markers were developed to fine map the Me3 gene. Analysis of 2272 individuals (F2 progenies) revealed that Me3 was located in a 45-kb DNA region between markers SSR784 and SSR339, in which there were three candidate genes. Among them, as a novel nucleotide binding site and leucine rich repeat (NBS-LRR) family gene, the DNA sequence of Capana09g000163 of pepper line ‘HDA149’ was 6348 bp in length, with a 2802-bp open reading frame encoding 933 amino acids, including NB-ARC and LRR domains. Tobacco transient expression assays demonstrated that expression of Capana09g000163 triggered a hypersensitive response (HR) in Nicotiana benthamiana leaves. Subcellular localization results showed that the Capana09g000163 protein was localized in the cell nucleus. Ectopic expression of Capana09g000163 in Arabidopsis significantly increased resistance against Meloidogyne incognita compared with the wild-type (WT) Arabidopsis. Furthermore, M. incognita was almost unable to develop in transgenic Arabidopsis expressing Capana09g000163. Taken together, we cloned the Me3 gene and verified that it induced resistance against M. incognita with the methods of map-based cloning and transgenic technology, which may be of great significance to pepper breeding for resistance against RKNs.

相似文章

热激或低温诱导辣椒产生2n花粉的细胞遗传学机理 [张新忠, 刘国俭, 闫立英, 赵永波, 常瑞丰, 吴禄平] 河北农业科学 2003,7 (3) 11-17
Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L. [Cao Ya-cong, Zhang Zheng-hai, Wang Li-hao, Sul Xiao-lei, Zhang Zhen-xian, Zhang Bao-xi] 农业科学学报 (英文) 2016,15 (4)
Effect of continuous negative pressure water supply on the growth, development and physiological mechanism of Capsicum annuum L. [Li Di, Long Huai-yu, Zhang Shu-xiang, Wu Xue-ping, Shao Hong-ying, Wang Peng] 农业科学学报 (英文) 2017,16 (9)
Cloning and characterization of CaGID1s and CaGAI in Capsicum annuum L. [Yan Cao, Zhenghai Zhang, Lihao Wang, Xiaolei Sui, Zhenxian Zhang, Baoxi Zhan] 农业科学学报(英文版) 2016,15 (4)
Effect of continuous negative pressure water supply on the growth, development and physiological mechanism of Capsicum annuum L. [Di Li, Huaiyu Long, Shuxiang Zhang, Xueping Wu, Hongbo Shao, Peng Wan] 农业科学学报(英文版) 2017,16 (9)
Mutation in the Agrobacterium hisI gene enhances transient expression in pepper [Dan Liu, Shengnan Zhao, Jubin Wang, Xi Zhang, Yadong Deng, Feng L] 园艺学报(英文版) 2023,10 (3)

导出引用

计量

文章访问数: 16

HTML全文浏览量: 0

PDF下载量: 0

Molecular cloning and functional analysis of the pepper resistance gene Me3 to root-knot nematode

相似文章

所属期刊

园艺学报(英文版)

推荐期刊

农业科学学报 (英文)