Jiaming Zhang;Mu Nan;Sui Yu-Xiang;Sun Xiaochun;Yan Guiqi

MU nan,SUI Yu-xiang,SUN Xiao-chun,YAN Gui-qin School of Life Science,Shanxi Normal University,Linfen,Shanxi 041004,Chin

elaeagnus mollis;alkaloids;total alkaloids;antimicrobial activity;staphylococcus aureus;bacillus subtili

[Object]To provide theoretical basis for the feasibility of developing total alkaloids in Elaeagnus mollis as botanical pesticide.[Method]Total alkaloids in the leaves of Elaeagnus mollis were extracted by ultrasonic-assisted method,five kinds of alkaloids were isolated and the anti-microbial activity of which were determined.[Result] Five kinds of alkaloids in Elaeagnus mollis had obvious anti-microbial effect on Bacillus subtilis,Staphylococcus aureus,V.ceratosperma and Verticillium dahliae while the bactriostasis on E.coli was unconspicuous,among which non-phenol tertiary amine alkaloids had the strongest antimicrobial activity and the MIC of Bacillus subtilis and Staphylococcus aureus were 0.4 mg/ml and 0.8 mg/ml respectively;when the density of non-phenol tertiary amine alkaloids was 3.2 mg/ml,the inhibition rates on V.ceratosperma and Verticillium dahliae were 72.7% and 93.4%;The alkaloids in Elaeagnus mollis had good resistant to heat,the antibacterial activity of which was not reduced after high temperature treatment;the antimicrobial activity of alkaloids was decreased through acidizing while the acidification antibacterial liquid had the strongest antimicrobial activity when the pH value was 7.[Conclusion]Total alkaloids in Elaeagnus mollis are expected to be broad-spectrum botanical pesticide.