中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 磁小体的碱性磷酸酶活性分析 中国农业大学学报 2018 (4) 8-13
Position: Home > Articles > Analysis of alkaline phosphatase activity of magnetosome Journal of China Agricultural University 2018 (4) 8-13

磁小体的碱性磷酸酶活性分析

作  者：
徐俊杰;王曼曼;刘凌子;李峰;田杰生
单  位：
淮北师范大学生命科学学院;中国农业大学生物学院/农业生物技术国家重点实验室
关键词：
磁小体;固相载体;碱性磷酸酶;磁性免疫ELISA;趋磁细菌
摘  要：
为测定磁小体的碱性磷酸酶活性及作为磁性免疫ELISA固相载体的可行性,设置不同显色时间、温度及pH条件,分析比较磁小体与商业化碱性磷酸酶的酶活。结果表明:商业化碱性磷酸酶显色时间较短,非常灵敏,但对温度和pH要求严格;微量的磁小体,需经过较长的显色时间,才能使显色剂显色,但温度和pH对磁小体的显色反应影响较小。通过不同的蛋白变性剂进行处理,表明磁小体的类碱性磷酸酶活性是其表面蛋白作用的结果。但磁小体微弱的显色能力,在磁性免疫ELISA检测时,没有显著影响。因此,磁小体可作为磁性免疫ELISA的固相载体,用于免疫检测。
译  名：
Analysis of alkaline phosphatase activity of magnetosome
作  者：
XU Junjie;WANG Manman;LIU Lingzi;LI Feng;TIAN Jiesheng;College of Life Science,Huaibei normal university;College of Biological Sciences/State Key Laboratory of Agricultural Biotechnology,China Agricultural University;
关键词：
magnetosome;;solid phase carrier;;alkaline phosphatase;;magnetic immune ELISA;;magnetotactic bacteria
摘  要：
An experiment was carried out to determine alkaline phosphatase-like activity in magnetosomes and whether the magnetosomes could act as the solid carrier of magnetic immune ELISA.The activity of the magnetosome and the commercial alkaline phosphatase was compared under different coloring time,temperature and pH.The results showed that the time used for the color development of commercial alkaline phosphatase was very short and sensitive.However,the temperature and pH had certain limitations.The trace of the magnetosome required long time in color development,temperature and pH had little effect on the color reaction of magnetosome.Moreover,the location of the magnetophilic alkaline phosphatase on magnetosome was preliminarily determined by the treatment of different protein denaturants.Based on these findings,it was concluded that the weak chromogenic ability of the magnetosome was not significantly affected when it was used for magnetic immunoassay ELISA.Therefore,the magnetosome could be used as a solid carrier for magnetic immune ELISA.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46