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当前位置: 首页 > 文章 > 耐冬山茶胚状体和胚性愈伤组织诱导研究 青岛农业大学学报(自然科学版) 2013,30 (3) 184-187+199
Position: Home > Articles > Induction of Somatic Embryogenesis and Embryogenic Callus from Camellia japonica Journal of Qingdao Agricultural University(Natural Science) 2013,30 (3) 184-187+199

耐冬山茶胚状体和胚性愈伤组织诱导研究

作  者：
杨凯
单  位：
青岛农业大学理学与信息学院
关键词：
耐冬山茶;幼胚;胚状体;胚性愈伤;激素
摘  要：
为快速扩大耐冬山茶的种质资源以满足园林应用的需求,本实验对不同发育时期、不同激素组合培养基中耐冬山茶幼胚中段和末段的胚状体和胚性愈伤诱导情况进行了探讨。结果表明,对于授粉后15~21周的幼果,胚状体的诱导率随幼胚发育成熟度提高基本呈现上升的趋势,但胚性愈伤的诱导效果与幼果成熟度之间无明显联系。在选用的8种激素组合中,1.0mg/L 6-BA和1.0mg/L 6-BA+0.2mg/L NAA的培养基对于胚状体诱导效果最佳,2.0/5.0mg/L 6-BA+0.5mg/L IBA的组合及1.0mg/L 6-BA培养基诱导胚性愈伤数量最多。此外,耐冬山茶胚状体在幼胚的末段部位发生较多,胚性愈伤在幼胚的中段部位较易形成。
译  名：
Induction of Somatic Embryogenesis and Embryogenic Callus from Camellia japonica
作  者：
YANG Kai;Science and Information College,Qingdao Agricultural University;
关键词：
Camellia japonica;;young embryo;;somatic embryogenesis;;embryogenic callus;;phytohormone
摘  要：
In order to satisfy the needs of landscape application by rapidly expand the germplasm resources of Naidong(Camellia japonica),somatic embryogenesis and embryogenic callus induced from young embryo of middle and lower segment under different development stage and phytohormone combination were studied.The results were as follows,in the young fruits of 15~21weeks after pollination,the inductivity of somatic embryogenesis presented upward tendency with the increase of the developmental embryo maturity,but the inductive effect of embryogenic callus had little relationship with developmental embryo maturity.Within the eight phytohormone combinations,the inductive effect of somatic embryogenesis were best on the culture medium with 1.0mg/L 6-BA,and 1.0mg/L 6-BA+0.2mg/L NAA.Meanwhile the maximum quantity of induced embryogenic callus were appeared on the culture medium with 2.0/5.0mg/L 6-BA+0.5mg/L IBA,and 1.0mg/L 6-BA.Furthermore somatic embryogenesis were easily induced on the lower segment of young embryo,and embryogenic callus more grew on the middle segment of young embryo in Naidong(C.japonica).

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