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当前位置: 首页 > 文章 > UC-HPLC法测定替米考星纳米结构脂质载体的包封率与载药量 畜牧与兽医 2019 (3) 56-61
Position: Home > Articles > Determination of encapsulation efficacy and loading capacity of tilmicosin loaded nanostructured lipid carriers by ultrafiltration centrifugation combined with high performance liquid chromatography Animal Husbandry & Veterinary Medicine 2019 (3) 56-61

UC-HPLC法测定替米考星纳米结构脂质载体的包封率与载药量

作  者：
张茜;SAHITO Benazir;李翎旭;彭麟;江善祥;郭大伟
单  位：
南京农业大学动物医学院
关键词：
替米考星;纳米结构脂质载体;超滤离心法;高效液相色谱法;包封率;载药量
摘  要：
旨在建立替米考星纳米结构脂质载体(TMS-NLCs)包封率与载药量的测定方法。采用超滤离心法分离TMS-NLCs与游离替米考星,并通过高效液相色谱(HPLC)仪测定包封率与载药量。结果:替米考星在1～20μg/m L范围内,峰面积与浓度呈良好线性关系(R2=0.999 9);日内及日间精密度RSD均小于5%;超滤膜吸附率、加样处理回收率以及超滤加样回收率均介于80%～120%之间。使用该法平行测定3批TMS-NLCs样品,所得平均包封率和载药量分别为(93.46±0.50)%和(9.23±0.08)%。结果表明,UC-HPLC法重复性好、准确度高、操作简便、快速易行,可用于TMS-NLCs包封率与载药量的测定。
译  名：
Determination of encapsulation efficacy and loading capacity of tilmicosin loaded nanostructured lipid carriers by ultrafiltration centrifugation combined with high performance liquid chromatography
作  者：
ZHANG Qian;SAHITO Benazir;LI Lingxu;PENG Lin;JIANG Shanxiang;GUO Dawei;College of Veterinary Medicine,Nanjing Agricultural University;
单  位：
ZHANG Qian%SAHITO Benazir%LI Lingxu%PENG Lin%JIANG Shanxiang%GUO Dawei%College of Veterinary Medicine,Nanjing Agricultural University
关键词：
tilmicosin;;nanostructured lipid carriers;;ultrafiltration centrifugation;;high performance liquid chromatography;;encapsulation efficacy;;loading capacity
摘  要：
This study was to establish a method of ultrafiltration centrifugation combined with high performance liquid chromatography(UC-HPLC) to determine the encapsulation efficacy and loading capacity of tilmicosin loaded nanostructured lipid carriers(TMS-NLCs).TMSNLCs and free tilmicosin were firstly separated using ultrafiltration centrifugation(UC),and then determined by HPLC.The results showed that the standard curve for HPLC analysis of TMS was a good linear pattern over a range from 1 to 20 μg/mL as indicated by R2=0.999 9.The precisions of the method as expressed by RSD% of the inter-day and intra-day assays were both less than 5%.The adsorption rate of the Millipore membrane,the recovery rate of the sample addition and the recovery rate of the ultrafiltration were between all 80%～120%.The encapsulation efficacy and loading capacity of the three batches of TMS-NLCs were(93.46±0.50) % and(9.23±0.08) %,respectively.Taken together,the UC-HPLC method was successfully established and might be used to determine the encapsulation efficacy and loading capacity of TMS-NLCs.

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