当前位置: 首页 > 文章 > EN01过表达对籽鹅卵泡颗粒细胞凋亡基因Bcl-2表达的影响 中国畜牧兽医 2013,40 (7) 10-13
Position: Home > Articles > Effects of ENO10verexpression with Adenovirus Vector on Bcl-2  in the Granulose Cell of Zi Goose China Animal Husbandry & Veterinary Medicine 2013,40 (7) 10-13

EN01过表达对籽鹅卵泡颗粒细胞凋亡基因Bcl-2表达的影响

作  者:
王江璐;计红;张伟宏;赫荣贺;郭景茹;郭丽;杨焕民
单  位:
黑龙江八一农垦大学动物科技学院
关键词:
籽鹅;EN01过表达;Bcl-2基因;颗粒细胞
摘  要:
本试验将原代培养的籽鹅卵泡颗粒细胞转染EN01腺病毒过表达载体,应用实时荧光定量PCR方法检测EN01过表达对颗粒细胞Bcl-2mRNA表达量的影响.结果发现EN01过表达使卵泡颗粒细胞Bcl-2mRNA表达量极显著增加(P〈O.01).提示EN01可能通过使Bcl-2基因表达量增加,从而抑制籽鹅卵泡颗粒细胞凋亡,促进卵泡的发育.
译  名:
Effects of ENO10verexpression with Adenovirus Vector on Bcl-2  in the Granulose Cell of Zi Goose
关键词:
Zi goose ENO1 overexpression Bcl-2 gene granulosa cells
摘  要:
The paper was to study the effects of ENO1 overexpression with adenovirus vector on Bel-2 in the granulose cell of Zi goose. Adenovirus vector with ENO1 were transfected to Zi goose primary cultured granulose cells, and Bcl-2 mRNA of granulose cells were detected by the method of Real-time fluorescence quantitative PCR. The results showed that ENO1 over expression made the Bcl-2 mRNA expression in the granulose cells significantly increase (P〈0.01). ENO1 overexpression made the primary culture follicle granulose cells in vitro improve Bcl-2 mRNA expression, which suggested that ENO1 might inhibit the apoptpsis of Zi ~oose ~zranulose cell.

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