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当前位置: 首页 > 文章 > 不同栽培区附子生物碱含量的比较研究 安徽农业科学 2014 (30) 10519-10520+10522
Position: Home > Articles > Study on the Content of Alkaloids of Different Cultivation Zones of Radix Aconiti Lateralis Preparata Journal of Anhui Agricultural Sciences 2014 (30) 10519-10520+10522

不同栽培区附子生物碱含量的比较研究

作  者：
舒晓燕;余马;侯大斌;郑仁林;李凤
单  位：
西南科技大学生命科学与工程学院
关键词：
附子;浸出物;总生物碱;双酯型生物碱
摘  要：
[目的]比较不同栽培区附子生物碱的含量。[方法]采用冷浸法测定水浸出物和醇浸出物含量,酸性染料比色法测定附子总生物碱含量,RP-HPLC测定双酯型生物碱含量。[结果]6个栽培区附子水溶性浸出物含量为10.272%~11.681%,醇溶性浸出物含量为1.621%~3.195%,总生物碱含量为0.987%~1.346%,中乌头碱含量为0.349~3.268 mg/g,乌头碱含量为0.406~0.841 mg/g,次乌头碱含量为1.284~3.134 mg/g。[结论]不同栽培区附子浸出物、总生物碱和双酯型生物碱含量差异较大,其结果为附子药材质量评价提供一定的理论参考。
译  名：
Study on the Content of Alkaloids of Different Cultivation Zones of Radix Aconiti Lateralis Preparata
作  者：
SHU Xiao-yan;YU Ma;HOU Da-bin;College of Life Science and Engineering,Southwest University of Science and Technology;
关键词：
Radix Aconiti Lateralis Preparata;;Extracts;;Alkaloids;;Diester diterpenoid alkaloids
摘  要：
[Objective] To research the content of alkaloids of different cultivation zones of Radix Aconiti Lateralis Preparata. [Method] According to the procedures recorded in the Appendix X A of 2010 Chinese Pharmacopoeia,extracts with cold soak of samples were measured. The content of total alkaloids and three kinds of diester diterpenoid alkaloids was measured respectively by acid-dye colorimetric and high performance liquid chromatography method. [Result] The content of water-soluble extracts was 10. 272%- 11. 681%,the content of 95% ethanol extracts was 1. 621%- 3. 195%,that of total alkaloids was 0. 987%- 1. 346%,that of mesaconitine was 0. 349%- 3. 268 mg / g,aconitine was 0. 406-0. 841 mg / g,hypaconitine was 1. 284- 3. 134 mg / g. [Conclusion] The content of extracts,total alkaloid and three kinds of diester diterpenoid alkaloids in six cultivation zones was rather different. The results provided a theoretical reference for the quality evaluation of Radix Aconiti Lateralis Preparata.

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