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当前位置: 首页 > 文章 > 厚颌鲂核型分析与DNA含量的测定 华中农业大学学报 2014,33 (1) 86-90
Position: Home > Articles > Karyotype and cellular DNA content analysis of Megalobrama pellegrini Journal of Huazhong Agricultural University 2014,33 (1) 86-90

厚颌鲂核型分析与DNA含量的测定

作  者：
易少奎;张新辉;杨坤;高泽霞;王卫民
单  位：
华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业部淡水生物繁育重点实验室
关键词：
厚颌鲂;染色体;核型;DNA含量
摘  要：
为了解厚颌鲂(Megalobrama pellegrini)的细胞遗传学特征,分析了厚颌鲂的染色体核型并对其DNA含量进行了测定。采用PHA和秋水仙素活体注射、肾细胞短期培养和空气干燥制片法,经Giemsa染色,对厚颌鲂染色体数目和核型进行研究,结果表明:厚颌鲂2n=48,染色体核型公式为24m+20sm+4st,臂数NF=92,具有1对特大染色体,未发现性染色体。以厚颌鲂外周血细胞为样本,鸡血细胞DNA为标准(2.30pg),使用流式细胞仪测定了厚颌鲂二倍体细胞的DNA含量,厚颌鲂DNA含量为鸡血的1.23倍,绝对含量为2.82pg。
译  名：
Karyotype and cellular DNA content analysis of Megalobrama pellegrini
作  者：
YI Shao-kui;ZHANG Xin-hui;YANG Kun;GAO Ze-xia;WANG Wei-min;CollegeofFisheries,Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture, Huazhong Agricultural University;
关键词：
Megalobrama pellegrini;;chromosome;;karyotype;;DNA content
摘  要：
In order to investigate the cytogenetic characters of Megalobrama pellegrini,the karyotype and DNA content were studied in the present study.The chromosome number and karyotype were analyzed by a routine method including intraperitoneal injection of PHA and colchicine,cultivation invivo,and slides preparation with air-drying and Giemsa staining.The results showed that M.pellegrini had a chromosome number of 48(2n=48),with a large and long chromosome pair and no heterosome,and the karyotype was 2n=24m+20sm+4st,NF=92.Erythrocyte nuclear DNA content of M.pellegrini was determined by flow cytometry using the chicken erythrocytes(DNA content is 2.30pg)as a standard.The DNA content of M.pellegrini is 2.82pg and the ratio of DNA content between M.pellegrini and chicken is 1.23.

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