中国农业期刊集成服务平台

当前位置: 首页 > 文章 > MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica 农业科学学报 (英文) 2016,15 (12)

Position: Home > Articles > MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica Journal of Integrative Agriculture 2016,15 (12)

MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica

作者：

Li Po;Gong Xiao-dong;Jia Hui;Fan Yong-shan;Zhang Yun-feng;Han Jian-min;Gu Shou-qin;Dong Jin-gao

单位：

Agr Univ Hebei, Mycotoxin & Mol Plant Pathol Lab, Baoding 071000, Peoples R China;Tangshan Normal Univ, Tangshan 063000, Peoples R China;Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Peoples R China

关键词：

Setosphaeria turcica;MAPK;conidiogenesis;HT-toxin;pathogenicity

摘要：

The mitogen-activated protein kinase (MAPK), a key signal transduction component in the MAPK cascade pathway, regulates a variety of physiological activities in eukaryotes. However, little is known of the role MAPK plays in phytopathogenic fungi. In this research, we cloned the MAPK gene STK1 from the northern corn leaf blight pathogen Setosphaeria turcica and found that the gene shared high homology with the high osmolality glycerol (HOG) MAPK gene HOG1 of Saccharomyces cerevisiae. In addition, gene knockout technology was employed to investigate the function of STK1. Gene knockout mutants (KOs) were found to have altered hyphae morphology and no conidiogenesis, though they did show similar radial growth rate compared to the wild-type strain (WT). Furthermore, microscope observations indicated that STK1 KOs did not form normal appressoria at 48 h post-inoculation on a hydrophobic surface. STK1 KOs had reduced virulence, a significantly altered Helminthosporium turcicum (HT)-toxin composition, and diminished pathogenicity on the leaves of susceptible inbred corn OH43. Mycelium morphology appeared to be significantly swollen and the radial growth rates of STK1 KOs declined in comparison with WT under high osmotic stress. These results suggested that STK1 affects the hyphae development, conidiogenesis, and pathogenicity of S. turcica by regulating appressorium development and HT-toxin biosynthesis. Moreover, the gene appears to be involved in the hypertonic stress response in S. turcica.

相似文章

高渗胁迫对玉米大斑病菌生长发育及SYKl表达的影响 [王梅娟, 李坡, 吴敏, 范永山, 谷守芹, 董金皋] 中国农业科学 2012,45 (19) 3965-3970
Melanin, DNA replication, and autophagy affect appressorium development in Setosphaeria turcica by regulating glycerol accumulation and metabolism [Guo Xiao-yue, Liu Ning, Liu Bing-hui, Zhou Li-hong, Cao Zhi-yan, Han Jian-min, Dong Jin-gao] 农业科学学报 (英文) 2022,21 (3)
Intelligent diagnosis of northern corn leaf blight with deep learning model [Pan Shuai-qun, Qiao Jing-fen, Wang Rui, Yu Hui-lin, Wang Cheng, Taylor, Kerry, Pan Hong-yu] 农业科学学报 (英文) 2022,21 (4)
Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize [Zhang Xiao-li, Si Bing-wen, Fan Cheng-ming, Li Hong-jie, Wang Xiao-ming] 农业科学学报 (英文) 2014,13 (4)
Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica, Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation, Invasive Growth, and Mating Behavior [Gu Shou-qin, Yang Yang, Li Po, Zhang Chang-zhi, Fan Yu, Zhang Xiao-yu, Tian Lan, Hao Zhi-min, Cao Zhi-yan, Gong Xiao-dong, Fan Yong-shan, Han Jian-min, Dong Jin-gao] 农业科学学报 (英文) 2013,12 (12)
Transcriptional regulation of secondary metabolism and autophagy genes in response to DNA replication stress in Setosphaeria turcica [Meng, Ya-nan, Zhang, Xin-jie, Zeng, Fan-li, Zhai, Wei-bo, Li, Pan, Hu, Jing-jing, Xiao, Sheng-lin, Hao, Zhi-min, Cao, Zhi-yan, Chen, Chuan, Dong, Jin-gao] 农业科学学报 (英文) 2023,22 (4)
MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica [Po Li, Xiaohua Gong, Hui Jia, Yingli Fan, Yunfeng Zhang, Zhimian Cao, Zhimin Hao, Jiahuai Han, Shuang Gu, Jingao Don] 农业科学学报(英文版) 2016,15 (12)
Proteomics Identification of Differentially Expressed Leaf Proteins in Response to Setosphaeria turcica Infection in Resistant Maize [Xiaoli Zhang, Bingwen Si, Chengming Fan, Hongjie Li, Xiaoming Wan] 农业科学学报(英文版) 2014,13 (4)
Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica, Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation, Invasive Growth, and Mating Behavior [Shuang Gu, Yang Yang, Po Li, Changzhi Zhang, Fan Yu, Xiaoyu Zhang, Tian Lan, Zhimin Hao, Zhimian Cao, Xiaohua Gong, Yingli Fan, Jiahuai Han, Jingao Don] 农业科学学报(英文版) 2013,12 (12)
Bioinformatic analysis and functional characterization of CFEM proteins in Setosphaeria turcica [WANG Jian-xia, LONG Feng, ZHU Hang, ZHANG Yan, WU Jian-ying, SHEN Shen, DONG Jin-gao, HAO Zhi-min] 农业科学学报 (英文) 2021,20 (9)

导出引用

计量

文章访问数: 10

HTML全文浏览量: 0

PDF下载量: 0

MAP kinase gene STK1 is required for hyphal, conidial, and appressorial development, toxin biosynthesis, pathogenicity, and hypertonic stress response in the plant pathogenic fungus Setosphaeria turcica

相似文章

所属期刊

农业科学学报 (英文)

推荐期刊

农业科学学报 (英文)