Position: Home > Articles > Hapten Design and Antibodies Development of Milbemycin Oxime
Chinese Journal of Pesticide Science
2005,7
(3)
285-288
Milbemycin Oxime半抗原设计及其抗体制备
作 者：
刘媛;刘贤进;余向阳;张存政;陈育如
单 位：
江苏省农业科学院农药生物生态实验室;南京师范大学生命科学学院
关键词：
milbemycin oxime;半抗原;酶联免疫吸附法(ELISA)
摘 要：
通过m ilbemycin oxime A4、A3(M ILO)肟羟基与琥珀酸酐反应,合成了半抗原M ILO-5-琥珀酰半酯,采用碳化二亚胺法将其与载体蛋白BSA偶联制备人工抗原。以此人工抗原免疫新西兰大白兔获得多克隆抗体,抗体效价达1∶12 800;用此抗体建立的间接竞争酶联免疫吸附(CI-ELISA)检测方法,线性范围在16.00~4 394.18 ng/mL,最低检测限(I10)为6.28 ng/mL。
译 名：
Hapten Design and Antibodies Development of Milbemycin Oxime
作 者：
LIU Yuan~(1,2), LIU Xian-jin~(*1), YU Xiang-yang~1, ZHANG Cun-zheng~1, CHEN Yu-ru~2(1. Pesticide Evaluation Lab , Jiangsu Academy of Agriculture Sciences, Nanjing 210014, China;2. College of Life Science, Nanjing Normal University, Nanjing 210097, China)
关键词：
milbemycin oxime; hapten; enzyme linked immunosorbent assay(ELISA)
摘 要：
To produce antigens susceptible to raise antibodies for milbemycin oxime A_4、A_3(MILO),the 5-O-succinoylmilbemycin-oxime were bound to bovine serum albumin (BSA) by the carbodiimide activation. Two New Zealand white rabbits were immunized with MILO-BSA and the titers of (antiserum) had reached 1∶12 800. A MILO-OVA-based CI-ELISA was conducted with the antiserum, the response range for milbemycin oxime was between 16.00～4 394.18 ng/mL and the limit of (detection) was 6.28 ng/mL.
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