Position: Home > Articles > STUDY ON MICROCARRIER CELL CULTURE TECHNIQUE FOR CULTURE OF PRIMARY CHICKEN EMBRYO FIBROBLASTS AND PROPAGTION OF NDV
Acta Agriculturae Shanghai
1991
(1)
1-7
细胞微载体培养技术用于鸡胚原代细胞培养及鸡新城疫病毒的增殖
作 者：
陈嘉棣;杨惠萍;杨颐;黎济申;赵洪兴;余鸣
单 位：
上海市农业科学院畜牧兽医研究所
关键词：
鸡胚原代细胞;微载体;培养技术;鸡新城疫Ⅰ系苗
摘 要：
以细胞微载体培养技术,用DEAE-Sephadex A 50,Cytodex 3二种微载体,对鸡胚原代细胞进行培养研究,并用在微载体上培养的鸡胚细胞感染鸡新城疫Ⅰ系毒,制备鸡新城疫Ⅰ系细胞苗免疫鸡,与普通细胞培养及鸡胚增殖病毒制备的鸡新城疫Ⅰ系苗比较,我们得到了在微载体培养系统有较高的细胞产量,较高的病毒滴度,培养物制备疫苗抗原性也好的结果。
译 名：
STUDY ON MICROCARRIER CELL CULTURE TECHNIQUE FOR CULTURE OF PRIMARY CHICKEN EMBRYO FIBROBLASTS AND PROPAGTION OF NDV
作 者：
Chen Jiadi Yang huiping Yang Yi Li jishen Zhao Hongxing Yu Ming(Animal Husbandry and V eterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 200030)
关键词：
Primary chicken embryo fibroblasts,Microcarrier Technique of culture Vaccine of NDV strain Ⅰ
摘 要：
Primary chicken embryo fibroblasts were cultured with microearrier cell culture technique using two kinds of microcarriers: DEAE-Sephadex A50 and Cytodex 3. The cultures of chicken embryo cells on Cytodex 3 were infected with NDV strain I, then NDV vaccine were prepared using these cultures. The NDV vaccines prepared from the cultures of chicken embryo cells on cytodex 3 were administered to chickens in parallel with the NDV vaccine prepared from cultures of chicken embryo ceils or chicken embryos. From microearrier cell culture system we got the good results with higher cell yield, higher virus titre as well as better antigenicity.
导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
