中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 家兔MITF基因部分外显子多态性的PCR-SSCP分析 江西农业大学学报 2012,34 (3) 161-165
Position: Home > Articles > PCR-SSCP Polymorphism of Parts of Exons in MITF Gene in Rabbit Acta Agriculturae Universitatis Jiangxiensis 2012,34 (3) 161-165

家兔MITF基因部分外显子多态性的PCR-SSCP分析

作  者：
何孟颉;李挺;王晓明;潘雨来;吴信生
单  位：
江苏省金陵种兔场;江苏省动物遗传繁育与分子设计重点实验室
关键词：
家兔;MITF;毛色;多态性;PCR-SSCP
摘  要：
小眼畸形相关转录因子MITF是色素细胞的发育成熟中的重要因子,可调控酪氨酸基因家族的表达,参与黑素生成的调控,从而影响动物的毛色。本实验采用PCR-SSCP技术,检测白色獭兔、海狸色獭兔和闽西南黑兔群体中家兔MITF基因的5个外显子和部分内含子区域的多态性,结果发现,引物1、2、5、7的扩增片段均未表现出多态,在获得的大小为255 bp的引物4扩增片段中发现一个SNP(C43T)位点,在各群体中表现为3种等位基因型AA、BB、AB。测序结果表明第4外显子扩增片段的突变位于5′端的内含子区域,不影响氨基酸序列。统计结果显示,白色獭兔群体不处在哈代温伯格平衡状态(P<0.05),其余群体则均处于平衡状态。闽西南黑兔群体表现出中度多态(0.5>PIC>0.25),其余群体为低度多态。
译  名：
PCR-SSCP Polymorphism of Parts of Exons in MITF Gene in Rabbit
作  者：
HE Meng-jie~(1,2),LI Ting~(1,2),WANG Xiao-ming~(1,2),PAN Yu-lai~3,WU Xin sheng~(1,2*) (1.Key Laboratory of Animal Heredity Breeding and Molecular Designing,Jiangsu Province,Yangzhou 225009,China;2.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;3. Jinling Rabbit Farm,Jiangsu Province,Jiangning 211103,China)
关键词：
rabbit;;Microphthalmia- Associated Transcription Factor(MITF);;hair color;;polymorphism;;PCR-SSCP
摘  要：
Microphthalmia-Associtated Transcription Factor plays an important role in the growth of pigment cells,which affects the furriery colors by controling the expression of tyrosinase gene family,and participating the regulation of melanin producting.ln this study,the polymorphism five exons and parts of introns of MITF gene were detected by PCR-SSCP in three rabbit populations include White Rex rabbit,Chinchilla Rex rabbit and Minxinan Black rabbit.The results indicated that the primers 1,2,5 and 7 did not display polymorphism,and a 255 bp polymorphism segment was obtained by primer 4.The polymorphism of the segment obtained by primer 4 was controlled by A and B alleles and formed three genotypes,namely AA, BB,AB.The result of cloning and sequencing showed that there was one single nucleotide mutation(C/T) at 43bp in 5'end of the extended segment,and it did not change the encoding amino acid.Statistical results showed that the MITF gene SSCP sites in White rabbite population was at Hardy-Weinberg disequilibrium (P<0.05),the other populations' were at Hardy-Weinberg equilibrium.PIC of Minxinan Black rabbite population was of medium polymorphism(0.5>PIC>0.25),others were of low polymorphism.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46