Shasha Luo;Qiang Gao;Shaojie Wang;Lei Tian;Qi Zhou;Xiujun Li;Chunjie Tia

Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102 (China); Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102 (China;College of Resources and Environment, Jilin Agricultural University, Changchun 130118 (China)

residue return;fertilizers;increased;fertilization;kmno4;h

Ecological stoichiometry provides the possibility for linking microbial dynamics with soil carbon (C), nitrogen (N), and phosphorus (P) metabolisms in response to agricultural nutrient management. To determine the roles of fertilization and residue return with respect to ecological stoichiometry, we collected soil samples from a 30-year field experiment on residue return (maize straw) at rates of 0, 2.5, and 5.0 Mg ha−1 in combination with 8 fertilization treatments: no fertilizer (F0), N fertilizer, P fertilizer, potassium (K) fertilizer, N and P (NP) fertilizers, N and K (NK) fertilizers, P and K (PK) fertilizers, and N, P, and K (NPK) fertilizers. We measured soil organic C (SOC), total N and P, microbial biomass C, N, and P, water-soluble organic C and N, KMnO4-oxidizable C (KMnO4-C), and carbon management index (CMI). Compared with the control (F0 treatment without residue return), fertilization and residue return significantly increased the KMnO4-C content and CMI. Furthermore, compared with the control, residue return significantly increased the SOC content. Moreover, the NPK treatment with residue return at 5.0 Mg ha−1 significantly enhanced the C:N, C:P, and N:P ratios in the soil, whereas it significantly decreased the C:N and C:P ratios in soil microbial biomass. Therefore, NPK fertilizer application combined with residue return at 5.0 Mg ha−1 could enhance the SOC content through the stoichiometric plasticity of microorganisms. Residue return and fertilization increased the soil C pools by directly modifying the microbial stoichiometry of the biomass that was C limited.