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当前位置: 首页 > 文章 > 假单胞菌DS-Ⅲ的脱氢酶活性与石油烃降解动力学特性 农业环境科学学报 2007,26 (02) 161-164
Position: Home > Articles > Dehydrogenase Activity of Pseudomonas DS-Ⅲ and Its Degradation Capability for Hydrocarbon Journal of Agro-Environment Science 2007,26 (02) 161-164

假单胞菌DS-Ⅲ的脱氢酶活性与石油烃降解动力学特性

作  者：
马宏瑞;赵敏;张景飞
单  位：
陕西科技大学资源与环境学院;南京大学地球科学系
关键词：
石油烃;微生物降解;脱氢酶活性;米氏方程
摘  要：
通过从油污土壤中分离筛选出的石油烃降解菌假单胞菌DS-Ⅲ,研究了在以烃为惟一碳源条件下该菌的生长特征、降解性与脱氢酶活性变化之间的关系。结果表明,DS-Ⅲ的脱氢酶活性滞后于其细胞生物量增长,适应后DS-Ⅲ的脱氢酶活性变化与柴油的降解趋势基本一致,DS-Ⅲ菌的对数生长期代时为4.05d,生长速率为0.25d-1。在培养温度32℃、pH6.5时,DS-Ⅲ的脱氢酶活性达到最高,在该条件下获得的米氏方程中,Km为159.16μg·L-1,Vmax为11.06μg·L-1·d-1。
译  名：
Dehydrogenase Activity of Pseudomonas DS-Ⅲ and Its Degradation Capability for Hydrocarbon
作  者：
MA Hong-rui1,2, ZHAO Min1, ZHANG Jing-fei2 (1. College of Resource & Environment, Shaanxi University of Science and Technology, Xianyang 712081, China;2. Department of Earth Science, Nanjing University, Nanjing 210093, China)
关键词：
hydrocarbon; biodegradation; dehydrogenase activity; Michaelis equation
摘  要：
Pseudomonas DS-Ⅲ, the bacteria degrading petroleum hydrocarbon, were isolated from the oil-contaminated soil, while the diesel oil was used as the sole source of carbon and energy. The characteristic of growth and degradation ability of DS-Ⅲ, along with its dehydrogenase activity, were then investigated and compared. The increase of dehydrogenase activity of DS-Ⅲ showed a lag compared with the corresponding biomass. After the domestication period, the dehydrogenase activity changed similarly with that the diesel oil degraded. The generation time and growth rate for DS-Ⅲ in logarithmic period reached 4.05 d and 0.25 d-1, respectively. The optimum culture condition was found at pH 6.5 and 30 ℃, in which Michaelis equation can be acquired by calculation, with Michaelis constant(Km) being 159.16 μg·L-1 and νmax 11.06 μg·L·d-1.

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