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当前位置: 首页 > 文章 > HPLC分析6种不同花色滇牡丹花瓣中花青素和黄酮 西部林业科学 2017 (6) 40-45
Position: Home > Articles > Analysis of Anthocyanins and Flavonols in six Different Colors of Petals of Paeonia delavayi by High Performance Liquid Chromatography Journal of West China Forestry Science 2017 (6) 40-45

HPLC分析6种不同花色滇牡丹花瓣中花青素和黄酮

作  者：
华梅;原晓龙;杨卫;陈剑;呼延丽;谭芮;杨宇明;王娟
关键词：
滇牡丹;花青素;黄酮;HPLC-DAD-ESI-MS~2
摘  要：
滇牡丹是牡丹野生种之一,具有非常丰富的颜色,有白色、黄色、黄绿色、橙色、红色(深红、紫红)、紫色和紫黑色等颜色。采用HPLC和HPLC-DAD-ESI-MS~2同时分析6种不同花色滇牡丹花瓣中花青素和黄酮成分,分析并鉴别出4种花青素化合物和7种黄酮化合物。研究表明:除黄色花瓣,其余5种颜色的花瓣中都含有相同种类的花青素,不同颜色花瓣中花青素的相对百分含量差距比较大。首次从滇牡丹粉色系花瓣中分析并鉴定出矢车菊素双葡萄糖苷(cyanidin-3,5-di-O-glucoside)、矢车菊素单葡萄糖苷(cyanidin-3-O-glucoside)、芍药素双葡萄糖苷(peonidin-3,5-di-O-glucoside)和芍药素单葡萄糖苷(peonidin-3-O-glucoside)。6种颜色滇牡丹花瓣中黄酮化合物的种类几乎相同,仅含量有差异。
译  名：
Analysis of Anthocyanins and Flavonols in six Different Colors of Petals of Paeonia delavayi by High Performance Liquid Chromatography
作  者：
HUA Mei;YUAN Xiao-long;YANG Wei;CHEN Jian;HU Yan-li;TAN Rui;YANG Yu-ming;WANG Juan;Yunnan Academy of Forestry;Key Laboratory for Conservation of Rare,Endangered &Endemic Forest Plants,State Forestry Administration/Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants;
关键词：
Paeonia delavayi;;anthocyanins;;flavonol;;HPLC-DAD-ESI-MS~2
摘  要：
Paeonia delavayi is one of the wild species of tree peony, which has rich colors including white,yellow,yellow-green,orange,red( dark-red,purple-red),purple and purple-black. In our study,the anthocyanin and flavonol from 6 kinds of Petals of Paeonia delavayi were analyzed by using high-performance liquid chromatography( HPLC) and high-performance liquid chromatography-diode array detection-mass spectrometry( HPLC-DAD-ESI-MS~2). 4 anthocyanin compounds and 7 flavonol compounds were tentatively identified. The results indicated that excepting the yellow petals,the other 5 kinds of petals have the same anthocyanins and the relative percentage of anthocyanins have significant difference in different colors of petals. Cyanidin-3,5-di-O-glucoside,cyanidin-3-O-glucoside, peonidin-3,5-di-O-glucoside and peonidin-3-O-glucoside were first analyzed in pink Paeonia delavayi. There were no obvious differences in the composition of flavonol among the 6 kinds of petals.

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