当前位置: 首页 > 文章 > 西南地区水稻细菌性条斑病菌的遗传多样性分析 江西农业大学学报 2011,33 (2) 264-269
Position: Home > Articles > Genotypic Diversity Analysis of Xanthomonas oryzae pv.oryzicola in Southwest China by Rep-PCR Acta Agriculturae Universitatis Jiangxiensis 2011,33 (2) 264-269

西南地区水稻细菌性条斑病菌的遗传多样性分析

作  者:
林琳;姬广海;马改转;王永吉;张丽辉
单  位:
云南农业大学
关键词:
水稻细菌性条斑病菌;小种;遗传多样性;Rep-PCR
摘  要:
选取云南、贵州、四川不同省市地区的水稻条斑病菌菌株141株,利用Rep-PCR技术筛选出多态性较好的引物对其DNA进行多态性分析。引物J3、ERIC、JELIR的扩增多态性较好,不同毒性菌株间的Rep-PCR指纹图谱差异很大。通过聚类分析显示,我国西南地区的水稻细菌性条斑病菌群体遗传分化明显,菌株的遗传族群与其致病性具有一定的相关性,强、中、弱毒性菌株大致归为不同的类群。因此,Rep-PCR技术可用于检测水稻细菌性条斑病菌的遗传变异与鉴定研究。
译  名:
Genotypic Diversity Analysis of Xanthomonas oryzae pv.oryzicola in Southwest China by Rep-PCR
作  者:
LIN Lin1,2,JI Guang-hai2,MA Gai-zhuan2,WANG Yong-ji2,ZHANG Li-hui2 (1.College of Agronomy and Biotechnology,YAU,Kunming 650201,China 2.Key Laboratory of Agricultural Biodiversity and Pests Control,Ministry of Education,YAU,Kunming 650201,China)
关键词:
Xanthomonas oryzae pv.oryzicola;race;genetic diversity;Rep-PCR
摘  要:
The genetic diversity of 141 strains of Xanthomonas oryzae pv.oryzicola from Yunnan,Guizhou and Sichuan provinces was analyzed polymorphously by Rep-PCR.Primer J3,ERIC and JELIR had better amplified polymorphism,different toxicity strains of the Rep-PCR fingerprints varied widely.Cluster analysis showed that significant genetic diversity exists within the population of Xanthomonas oryzae pv.oryzicola in Southwest China.There was a high degree of correlation between the genetic strains populations of and their pathogenicity.Strains of strong,middle or weak toxicity were roughly classified into different genetic populations.The result suggests that Rep-PCR technique can be used for detecting genetic variation among strains of Xanthomonas oryzae pv.oryzicola and identification of strains.

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