Lipase catalyzed synthesis of structured phospholipids rich in ARA

WANG Xiang;WEI Fang;DONG Xu-yan;CAO Ying-ying;LIU Si-lei;LV Xin;CHEN Hong;Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Hubei Key Laboratory of Lipid Chemistry and Nutrition;

Structured phospholipid;;Arachidonic acid;;Response surface methodology;;Lipase;;Transesterification

To artificially produce polyunsaturated fatty acids( PUFA) using soybean powder,lipase lipozyme RM IM was selected as biocatalyst to catalyze transesterification of soy lecithin and arachidonic acid ethyl ester( ARA- EE) to synthesize structured phospholipids enriched with ARA in hexane solvent system. Effects of 4 variations( reaction temperature,reaction time,substrate molar ratio and lipase dosage) on the reaction were investigated. By single factor experiments,the conditions of structured phospholipids synthesis were optimized by response surface methodology( RSM). And the optimum conditions were as follows: reaction temperature 55. 6℃,reaction time 25. 1h,substrate molar ratio 1∶ 10. 1,and 15. 3% lipase dosage. Under these conditions,the relative percentage of ARA in structured phospholipids products reached 15. 42%,and the yield of ARA structured phospholipids was13. 26%. In addition,the PC species in the structured phospholipids were identified by mass spectrometry( MS).