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Position: Home > Articles > Comparative Studies of Enzymatic Hydrolysis of Chicken Embryo Protein by Different Proteases MEAT RESEARCH 2011 (9) 21-24

不同酶酶解鸡胚蛋白工艺比较研究

作  者:
梁琼;张培正;高伟;孙合美
单  位:
海口经济学院工程技术学院;云南农业大学食品科学与工程学院
关键词:
鸡胚;蛋白酶;酶解;多肽
摘  要:
以鸡胚蛋白为底物,通过单因素及正交试验,确定菠萝蛋白酶和胰蛋白酶对鸡胚蛋白水解的最佳工艺。结果表明:菠萝蛋白酶酶解鸡胚蛋白最佳工艺条件为底物质量分数6%、酶解温度55℃、pH5、酶解时间6h、酶与底物质量比([E/S])为1.5%,水解度可达45.53%;胰蛋白酶酶解鸡胚蛋白最佳工艺条件为底物质量分数6%、酶解温度55℃、pH5、酶解时间6h、酶与底物质量比([E/S])为1.5%,水解度可达38.17%。菠萝蛋白酶和胰蛋白酶在最佳水解条件下水解度分别为45.53%、38.17%,可溶性蛋白含量分别为20.15、22.69mg/mL,多肽含量分别为62.55、50.26mg/mL。菠萝蛋白酶比胰蛋白酶更适宜酶解鸡胚蛋白。
译  名:
Comparative Studies of Enzymatic Hydrolysis of Chicken Embryo Protein by Different Proteases
作  者:
LIANG Qiong1,ZHANG Pei-zheng2,GAO Wei2,SUN He-mei2(1.College of Engineering and Technology,Haikou College of Economics,Haikou 570203,China; 2.College of Food Science and Engineering,Yunnan Agricultural University,Kunming 650201,China)
关键词:
chicken embryo;protease;hydrolysis;peptides
摘  要:
The optimal process conditions for hydrolyzing chicken embryo protein by trypsin or bromelain were determined using one-factor-at-a-time combined with orthogonal array design method.Under the optimal process conditions,the degree of hydrolysis of chicken embryo protein by trypsin and bromelain were 45.53% and 38.17%,and the resulting hydrolyates contained 20.15 mg/mL and 22.69 mg/mL soluble protein and 62.55 mg/mL and 50.26 mg/mL polypeptides,respectively.These results demonstrate that bromelain is more suitable to hydrolyze chicken embryo protein than trypsin.

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