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当前位置: 首页 > 文章 > 桂花花粉超低温保存和花粉管萌发研究 甘肃农业大学学报 2014 (1) 105-110+116
Position: Home > Articles > Study on pollen cryopreservation and pollen germination processes of different cultivars of Osmanthus fragrans Journal of Gansu Agricultural University 2014 (1) 105-110+116

桂花花粉超低温保存和花粉管萌发研究

作  者：
李稚;陈洁;张洪伟;王贤荣
单  位：
南京林业大学森林资源与环境学院
关键词：
桂花;花粉活力;花粉管;超低温;荧光显微
摘  要：
试验于桂花生长的初花期至盛花初期采集不同品种的桂花花粉,干燥后将花粉保存于超低温液氮中,采用反复冻存和常温解冻30min的方法于第15、30、60、90、180、360天时,用离体培养基萌发法测定花粉活力.结果表明:桂花花粉活力并不高,新鲜花粉活力最高只有31.4%,液氮贮藏可延长其活力,活力保持在10%~20%之间,贮藏180d后花粉基本失活.对比人工授粉和自然授粉花粉管萌发过程研究发现:自然授粉条件下,花粉在开花第3天部分开始萌发,第5~6天有花粉管进入子房;人工授粉后2h柱头上有少量花粉萌发,4h形成花粉管并进入柱头生长,24h到达子房顶部;人工授粉比自然授粉花粉萌发率高.
译  名：
Study on pollen cryopreservation and pollen germination processes of different cultivars of Osmanthus fragrans
作  者：
LI Zhi;CHEN Jie;ZHANG Hong-wei;WANG Xian-rong;College of Forestry Resources and Environment,Nanjing Forestry University;
关键词：
Osmanthus fragrans;;pollen viability;;pollen tube;;cryopreservation;;fluorescence microscopy
摘  要：
Osmanthus fragrans is very famous flower with rich cultivars in China,but a few seeds were found in nature.The breeding system structures between different cultivars were not studied systematically and the pollen germination obstacles were thought as one important reason.The experiment on different varieties of pollen cryopreservation of osmanthus fragrans showed that the pollen vitality of Osmanthus fragrans was not high,fresh pollen vitality could get setting rate 31.4%.Liquid nitrogen storage could prolong its vitality and vigor remain between 10%and 20%,pollen almost dead after 180d.Study on pollen germination processes found,in natural pollinations,pollen germination started 3dafter flowering;pollen tubes reached ovary about 5~6dafter flowering.After 2hby artificial pollination,pollen germination started on stigma;after 4h,pollen tubes got into stigma;after 24h,pollen tubes reached ovary.And there was a higher pollen germination rate by artificial pollination than by natural pollination.

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