中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 牛外周血单核巨噬细胞的分离、培养与鉴定 畜牧与饲料科学 2013,34 (5) 21-22,25
Position: Home > Articles > 牛外周血单核巨噬细胞的分离、培养与鉴定 Animal Husbandry and Feed Science 2013,34 (5) 21-22,25

牛外周血单核巨噬细胞的分离、培养与鉴定

作  者：
陈玉惠;周伟光;高晶;高龙;冀显亮;希尼尼根
单  位：
农业部动物疾病临床诊疗技术重点实验室
关键词：
牛;单核巨噬细胞;分离;鉴定;流式细胞术;形态学观察
摘  要：
试验旨在为深入研究单核巨噬细胞的生物学特性和免疫学功能提供材料.应用牛淋巴细胞分离液从牛外周血中分离牛外周血单个核细胞,用含10%胎牛血清的RPMI1640营养液培养5d,在倒置显微镜下观察细胞形态,采用流式细胞术检测单核巨噬细胞表面特征性标志CD14.结果表明,获得的贴壁细胞具备巨噬细胞的形态学特征及免疫表型.通过形态学观察和流式细胞术鉴定可知,该试验成功获得了牛外周血单核巨噬细胞.
单  位：
College of Veterinary Medicine of Inner Mongolia Agricultural University,Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease of Ministry of Agriculture,Hohhot 010018,China
关键词：
bovine%monocyte macrophage%isolation%identification%flow cytometry%morphological observation
摘  要：
To provide materials for further study on the biological characteristics and immunological functions of monocyte macrophage,bovine peripheral blood mononuclear cells were isolated by using bovine lymphocyte separation medium and incubated in RPMI 1640 medium supplemented with 10% fetal bovine serum for 5 days. The cell morphology was observed by inverted microscope and surface characteristic marker CD14 of monocyte macrophages was detected by using flow cytometry. The results showed that the obtained adherent cells had the morphological characteristics and immunophenotype of macrophage. Through the morphological observation and identification by flow cytometry, bovine peripheral blood monocyte macrophage was successfully obtained.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46