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Position: Home > Articles > Study on Transferring Gene of Salt Tolerance and Drought Resistance into Corn Inbred Line by Pollen Tube Path Journal of Shanxi Agricultural Sciences 2011,39 (5) 392-394

用花粉管通道法将抗旱耐盐基因导入玉米自交系的研究

作  者:
董春林;张明义;林忠平;张彦芹;杨丽莉;梁改梅;孙洁
单  位:
北京大学生命科学学院;山西省农业科学院旱地农业研究中心
关键词:
玉米;花粉管通道技术;海藻糖合酶基因(TPS);转基因
摘  要:
采用改进的花粉管通道法,将海藻糖合酶基因(TPS)导入玉米自交系郑58中。3~5叶期时喷雾Basta(0.7%)溶液筛选,进行PCR检测,结果表明,目的基因已导入转化植株并整合到植株染色体上。田间初步抗旱性检测显示,转化植株(株系)的抗旱性高于对照植株。应用改进的花粉管通道法导入抗旱目的基因,可以得到玉米抗旱新资源,是一种切实有效的抗旱育种新方法。
译  名:
Study on Transferring Gene of Salt Tolerance and Drought Resistance into Corn Inbred Line by Pollen Tube Path
作  者:
DONG Chun-lin1,ZHANG Ming-yi1,LIN Zhong-ping2,ZHANG Yan-qin1, YANG Li-li1,LIANG Gai-mei1,SUN Jie1(1.Research Center of Arid Farming,Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China; 2.College of Life Sciences,Beijing University,Beijing 100871,China)
关键词:
corn;pollen tube path;trehalose synthase gene;transgenic
摘  要:
Using the improved pollen tube path method,trehalose synthase gene(TPS) was transferred into corn inbred line Zheng 58.In the 3~5 leaf stage,the selection of spraying Basta(0.7%)solution was combined with PCR test,the new gene had been imported into the plants and integrated into the plant chromosomes.The initial drought-tolerance investigation in fields confirmed that the drought-tolerance of transgenic plants was higher than the non-transformed.The results showed that the new corn drought resistance germplasm could be gained by pollen tube path,and it is a new feasible and effective way for corn drought resistance breeding.

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