Position: Home > Articles > Cryopreservation of Murine Embryo Fibroblast and New Bovine Testicular Fibroblast
Acta Agriculturae Boreali-occidentalis Sinica
2000,9
(3)
5-8
小鼠胎儿和牛睾丸成纤维细胞的冷冻保存
作 者：
安立龙;杨奇;窦忠英;雷安民;杨春荣;邱怀;高志敏
单 位：
西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放实验室!陕西杨陵712100,西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放实验室!陕西杨陵712100,西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放实验室!陕西杨陵712100,西北农林科技大学家畜生殖内?;西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放实验室
关键词：
小鼠胎儿;牛睾丸;成纤维细胞;冷冻保存
摘 要：
研究了冷冻保护液、平衡时间、降温速度、解冻液以及解冻方式对冷冻小鼠胎儿成纤维细胞和牛睾丸成纤维细胞活力的影响。结果表明 ,对小鼠胎儿成纤维细胞和犊牛睾丸成纤维细胞进行冷冻 ,冷冻液 ( 0 .4 %BSA) +1 .5mol/ L乙二醇 +0 .1 mol/ L蔗糖的 PBS液 )的冷冻效果优于冷冻液 ( 1 0 %的 NBS+1 0 %DMSO的 DMEM液 )。用冷冻液 对小鼠胎儿成纤维细胞和犊牛睾丸成纤维细胞进行冷冻 ,平衡时间可从 2 .5h缩短到 0 .5h;冷冻细管降温速度 ( -6.8～ -3 7℃ )可以从 0 .6℃ / min提高到 1 .2℃ / min;诱发结晶可以省略。 3种解冻液解冻细胞活力排序为 :解冻液 ( 0 .4 %BSA+0 .1 mol/ L蔗糖的 PBS) >解冻液 ( 1 0 %NBS的 DMEM) >解冻液 ( PBS)。用冷冻液 对 MEF和 BTF细胞冷冻过程中 ,无需用培养液制备细胞悬液反复离心
译 名：
Cryopreservation of Murine Embryo Fibroblast and New Bovine Testicular Fibroblast
作 者：
AN Li long,YANG Qi, DOU Zhong ying,LEI An min, YANG Chun rong,QIU Huai, GAO Zhi min (The College of Animal Science and Veterinary Medicine, NWSUAF,Yangling Shaanxi 712100)
关键词：
Murine embryos; New bovine testicular; Fibroblast cell; Cryopreservation
摘 要：
Cryopreservation of murine embryo fibroblast and new bovine testicular fibroblast were investigated in this paper.The results showed as follows: The vitality of murine embryo fibroblast(MEF) cells and new bovine tisticular fibroblast(NBTF) cells exposed to the freezing media Ⅱ(PBS supplemented 0.4% BSA,1.5 mol/L ethylene glycol(EG),0.1 mol/L sucrose) higher than freezing media Ⅰ(10% NBS+10%DMSO+DMEM); When NBTF and MEF were freezed in media Ⅱ,the balanced time can be reduced from 2.5 h to 0.5 h without influence on their vitality. Moreover,freezing rate can be increased from 0.6℃/min to 1.2℃/min,induced ice did not influence on their vitality.On the vitality of NBTF cells and MEF cells,thawing media Ⅱ(PBS supplemented 0.4% BSA,0.1 mol/L sucrose)>thawing media Ⅲ(DMEM supplemented 10% NBS)>thawing media ⅠPBS.
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