作 者：
沈锦玉;Thompson K D;Adams A;Galina Jeney;钱冬;沈智华
单 位：
Aquaculture and Irrigation;Institue of Aquaculture University of Stirling;Research Institute for Fisheries;Hungary;Institue of Aquaculture;浙江省淡水水产研究所;University of Stirling;H-5540;Szarvas;UK;P.O.Box 47;Stirling;FK9 4LA
摘 要：
Aeromonas hydrophila septicemia was a serious infectious disease occurred in China.The majority harmed fish was crucian carp(Carassius auratus gibelio).It was reported that immune prevention was the effect measure to prevent this disease using A.hydrophila bacterin.So it is necessary to develop monoclonal antibody(McAb) against the IgM from Crucian carp.In this study,the purification of serum immunoglobulin from Crucian carp was carried out by using Goat IgG Agarose.The concentration of purified IgM was 2.5 mg·mL~(-1).By using hybridoma-monoclonal antibody technology,hybridoma cell line secreted McAb against the IgM from crucian carp had been established and the characteristics of mab8 had been described.In isotyping analysis,the results showed that it belong to IgG_1,it was able to recognize the heavy chain of the IgM on western blot,and did not has any cross reaction with the Ig from 19 different fish species including common carp(Cyprinus carpio),bighead carp(Aristichthys nobilis),grass carp(Ctenopharhyngodon idella) and bream(Abramis brama) etc.The results demonstrated that McAb was highly specific and sensitive to the Ig of Crucian carp,and had the great potential to be used for analyzing the structure of crucian carp IgM,monitoring the immunity level of cultured Crucian carp and pathogen diagnosis.
译 名：
Production and characterization of monoclonal antibodies against Carassius auratus gibelio IgM
作 者：
SHEN Jin-yu~1,Thompson K D~2,Adams A~2,Galina Jeney~3,QIAN Dong~1,SHEN Zhi-hua~1(1.Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China;2.Institue of Aquaculture,University of Stirling,Stirling,FK9 4LA,UK;3.Research Institute for Fisheries,Aquaculture and Irrigation,Szarvas,P.O.Box 47,H-5540,Hungary)
关键词：
Carassius auratus gibelio;immunoglobulin;monoclonal antibody
摘 要：
Aeromonas hydrophila septicemia was a serious infectious disease occurred in China.The majority harmed fish was crucian carp(Carassius auratus gibelio).It was reported that immune prevention was the effect measure to prevent this disease using A.hydrophila bacterin.So it is necessary to develop monoclonal antibody(McAb) against the IgM from Crucian carp.In this study,the purification of serum immunoglobulin from Crucian carp was carried out by using Goat IgG Agarose.The concentration of purified IgM was 2.5 mg·mL~(-1).By using hybridoma-monoclonal antibody technology,hybridoma cell line secreted McAb against the IgM from crucian carp had been established and the characteristics of mab8 had been described.In isotyping analysis,the results showed that it belong to IgG_1,it was able to recognize the heavy chain of the IgM on western blot,and did not has any cross reaction with the Ig from 19 different fish species including common carp(Cyprinus carpio),bighead carp(Aristichthys nobilis),grass carp(Ctenopharhyngodon idella) and bream(Abramis brama) etc.The results demonstrated that McAb was highly specific and sensitive to the Ig of Crucian carp,and had the great potential to be used for analyzing the structure of crucian carp IgM,monitoring the immunity level of cultured Crucian carp and pathogen diagnosis.
