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当前位置: 首页 > 文章 > 降胆固醇乳酸菌的筛选及对大鼠血脂的影响 食品科学 2011,32 (21) 223-228
Position: Home > Articles > Screening of Cholesterol-reducing Lactic Acid Bacteria and Its Effect on Blood Lipids in Rats FOOD SCIENCE 2011,32 (21) 223-228

降胆固醇乳酸菌的筛选及对大鼠血脂的影响

作  者：
杨琴;胡颖;甘盛力;朱秋劲;何腊平
单  位：
贵州省乳品工程技术研究中心;贵州大学生命科学学院
关键词：
乳酸菌;降胆固醇;筛选;发酵;肠球菌属;动物模型
摘  要：
对分离自发酵品及肠道排泄物的乳酸菌进行降胆固醇体外筛选及体内评价。结果显示:所分离得到的菌株中,对MRS-CHOL培养基胆固醇降解率最高的是R-2菌株(26.97±0.51)%和I-2菌株(23.72±0.41)%;R-2和I-2菌株对酸(pH3.0)有一定耐受性,在0.3g/100mL胆盐质量浓度条件下培养6h后,I-2的活菌数为1.1×104CFU/mL,R-2的活菌数为2.2×106CFU/mL。与对照组相比,采用I-2菌株和R-2菌株饲喂高脂大鼠,均可明显抑制大鼠总胆固醇(TC)(P<0.01)和低密度脂蛋白胆固醇(LDL-C)(P<0.05)升高;经鉴定,I-2菌株和R-2菌株分别为海氏肠球菌(Enterococcus hirae)和屎肠球菌(Enterococcus faecium)。
译  名：
Screening of Cholesterol-reducing Lactic Acid Bacteria and Its Effect on Blood Lipids in Rats
作  者：
YANG Qin1,HU Ying1,GAN Sheng-li1,ZHU Qiu-jin1,2,3,,HE La-ping1,2,3,HU Ping1,2,3,ZHU Jian-rong3,4 (1.College of Life Sciences,Guizhou University,Guiyang 550025,China;2.Food Science and Engineering Research Center,Guizhou University,Guiyang 550025,China;3.Guizhou Province Dairy Engineering Technology Research Center,Guiyang 550018,China;4.Guiyang Sanlian Dairy and Food Co.Ltd.,Guiyang 550018,China)
关键词：
lactic acid bacteria;cholesterol-reducing;screening;fermentation;Eenterococcus;animal model
摘  要：
In this study,lactic acid bacteria with the ability to degrading cholesterol were screened from fermented products and human feces.Strains R-2 and I-2 had the highest cholesterol degradation rate in the MRS-CHOL culture medium among the selected strains,reaching(26.97 ± 0.51)% and(23.72 ± 0.41)%,respectively.Both strains exhibited good tolerance to low pH(3.0).In the presence of 0.3 g/100 mL bile salt,the viable cell counts of I-2 and R-2 after 6 hours of cultivation were 1.1 × 104 CFU/mL and 2.2 × 106 CFU/mL,respectively.Moreover,R-2 and I-2 could inhibit the increase of TC(P < 0.01) and LDL-C(P <0.05) to some extent.R-2 and I-2 were identified as Enterococcus faecium and Enterococcus hirae,respectively by bacterial identification technology.

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