Position: Home > Articles > Determination of the residual quantity of aflatoxin B1 in the samples from broiler tissues using high performance liquid chromatography
Heilongjiang Animal Science and Veterinary Medicine
2015
(19)
281-283+287
高效液相色谱法对肉鸡组织样品中黄曲霉毒素B1残留量的测定
作 者：
姚婉;秦顺义;王昕;赵方红;刘丽娜
单 位：
天津农学院动物科学与动物医学学院;南京农业大学动物医学院;青岛农业大学动物科技学院
关键词：
肉鸡组织样品;柱前衍生-高效液相色谱(HPLC);黄曲霉毒素B1(AFB1);检出限;回收率
摘 要：
为了检测肉鸡组织中黄曲霉毒素B1(AFB1)的残留量,试验采用柱前衍生-高效液相色谱(HPLC)的分析方法,并用该方法对样品进行检测。结果表明:AFB1在0.5~50.0μg/L范围内具有良好的线性关系,相关系数大于0.999,检出限为0.1μg/kg,信噪比(S/N)=3∶1。在0.5μg/kg、1.0μg/kg、2.0μg/kg 3个添加水平下肝脏组织平均回收率为91.0%~107.0%,肌肉组织平均回收率为94.0%~105.4%,相对标准偏差(RSD)分别为0.8%~1.7%、1.2%~2.0%。说明本方法具有灵敏性、准确性和可重复性,可对动物组织样中AFB1残留进行定性和定量检测。
译 名：
Determination of the residual quantity of aflatoxin B1 in the samples from broiler tissues using high performance liquid chromatography
作 者：
YAO Wan;QIN Shun-yi;WANG Xin;ZHAO Fang-hong;LIU Li-na;ZHANG Lan-xiu;College of Animal Science and Veterinary Medicine,Tianjin Agricultural University;Yanshan County Bureau of Animal Husbandry and Aquatic Products of Hebei Province;
关键词：
samples from broiler tissues;;pre-column derivatization;;high performance liquid chromatography(HPLC);;aflatoxin B1(AFB1);;detection limit;;recovery rate
摘 要：
To detect residual quantity of aflatoxin B1( AFB1) in broiler tissues,the method of a high performance liquid chromatography( HPLC) with pre- column derivatization was used to detect the samples from broiler tissues. The results showed that the calibration curves of AFB1 had good linearity relationship in the range from 0. 5 to 50. 0 μg / L,and the correlation coefficient was greater than 0. 999. The limits of detection were 0. 1 μg / kg with a signal to noise ratio of 3∶1. The average recovery rates of AFB1 in liver tissue were from 91. 0% to 107. 0%at three adding levels of 0. 5 μg / kg,1. 0 μg / kg and 2. 0 μg / kg for AFB1,while the average recovery rates of AFB1 in muscle tissue were from94. 0% to 105. 4%,and the values of relative standard deviation( RSD) for the average recovery rates of AFB1 in liver tissue and muscle tissue were from 0. 8% to 1. 7% and from 1. 2% to 2. 0%,respectively. The results indicate that the method is suitable for qualitative and quantitative detection of aflatoxin B1 residues in the samples from animal tissues due to its sensitivity,accuracy and repeatability.
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