中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 芥菜开花负调因子SVP及FLC同源互作域筛选和作用强度分析 园艺学报 2013,40 (4) 675-684
Position: Home > Articles > Evaluation of Acting Domain and Strength Mediating the Protein Self-interactions of SVP and FLC in Brassica juncea Acta Horticulturae Sinica 2013,40 (4) 675-684

芥菜开花负调因子SVP及FLC同源互作域筛选和作用强度分析

作  者：
汤青林;丁宁;李念祖;王志敏;宋明
单  位：
西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室;西南大学园艺园林学院
关键词：
芥菜;截短体;SVP;FLC;酵母双杂交
摘  要：
为探明芥菜开花负调因子SVP、FLC自身聚合的分子机制及其蛋白作用模式,利用酵母双杂交体系,分别对SVP、FLC蛋白自身聚合及其作用强度进行研究。结果表明:酵母菌Y187转化子Y187-pGADT7SVP和Y187-pGADT7SVP2~5均能与酵母菌Y2HGold转化子Y2HGold-pGBKT7SVP融合,并可在选择性固体培养基QDO/X/A上长出蓝色菌落,而Y187-pGADT7SVP1×Y2HGold-pGBKT7SVP不能在QDO/X/A生长。说明SVP蛋白能自身聚合,且与截短体SVP2~5同源结合,SVP蛋白自身聚合需要核心作用域K域参与。尽管MI域不能单独介导SVP自身聚合,但它的存在却能使SVP自身聚合作用增强,C域有可能会削弱该作用。同时,Y2HGold-pGBKT7FLC和Y2HGold-pGBKT7FLC2~5也能与Y187-pGADT7FLC融合,同时激活报告基因AUR1-C、HIS3、ADE2、MEL1,FLC能与截短体FLC2~5同源互作。K域是FLC蛋白自身聚合必须的,I域会增强这一作用。SVP和FLC的核心作用域K域均由K1、K2和K3亚域组成,形成3个经典的α螺旋,K域有9个高度保守的氨基酸位点及蛋白互作的结构模体(亮氨酸拉链)。
译  名：
Evaluation of Acting Domain and Strength Mediating the Protein Self-interactions of SVP and FLC in Brassica juncea
作  者：
TANG Qing-lin,DING Ning*,LI Nian-zu,WANG Zhi-min,and SONG Ming**(College of Horticulture and Landscape Architecture,Southwest University;Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education;Key Laboratory of Olericulture,Chongqing 400715,China)
关键词：
Brassica juncea;truncated forms;SVP;FLC;yeast two-hybrid system
摘  要：
For further study on the molecular mechanism and interaction model of SVP and FLC protein homologous dimerization in flowering control in Brassica juncea Coss.(mustard),the selfinteractions of SVP and FLC were detected by the yeast two-hybrid system.The yeast stains of pGADT7SVP or pGADT7SVP2–5 could mate with pGBKT7SVP,which grew on selective agar plates QDO/X/A(SD/-Ade/-His/-Leu/-Trp/X-α-Gal/AbA)with blue stains.However,Y187-pGADT7SVP1 and Y2HGold-pGBKT7SVP could not mate into zygote diploids to grow on selective plates DO/X/A.Theresults showed that SVP or SVP2–5 truncated forms could act with SVP itself to combine and form homodimers.K domain of SVP was the key amino acid region to independently mediate and determine the homologous dimerization.MI-domain of SVP alone could not induce the self-interactions of SVP,but enhance the strength of homologous interactions.However,C-domain of SVP could weaken the protein self-interaction strength.The yeast stains of pGBKT7FLC2–5 and pGADT7FLC could mate into zygotes and grew on selective agar plates QDO/X/A with blue stains.The DNA-BD and AD were brought into proximity to activate transcription of four independent reporter genes(AUR1-C,HIS3,ADE2,MEL1).FLC2–5 truncated forms and FLC protein could act with each other to form homodimers.It also indicated that K domain of FLC may play an important role in mediating the FLC homodimers.However,I-domain of FLC could strengthen the protein self-interactions.Alignment analysis of K domain sequence showed that K domain was consist of three subdomains(K1,K2 and K3)and formed three helixes.Nine high conservative amino acids existed in K domain.Leucine zippers,protein interaction motifs,lied in K domain.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46