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当前位置: 首页 > 文章 > 基于rDNA ITS1和ITS2序列的褐飞虱、白背飞虱和灰飞虱的分子鉴定 昆虫学报 2009,52 (11) 1266-1272
Position: Home > Articles > Molecular identification of Nilaparvata lugens (Stl),Sogatella furcifera (Horvath) and Laodelphax striatellus (Fallén)(Homoptera:Delphacidae) based on rDNA ITS1 and ITS2 sequences Acta Entomologica Sinica 2009,52 (11) 1266-1272

基于rDNA ITS1和ITS2序列的褐飞虱、白背飞虱和灰飞虱的分子鉴定

作  者：
刘玉娣;林克剑;韩兰芝;侯茂林
单  位：
中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
关键词：
褐飞虱;白背飞虱;灰飞虱;rDNA;ITS1;ITS2;特异引物;分子鉴定
摘  要：
本研究测定了褐飞虱Nilaparvata lugens、白背飞虱Sogatella furcifera和灰飞虱Laodelphaxstriatellus的rDNA ITS1和ITS2的序列,以探讨这3种稻飞虱的分子鉴定方法。3种飞虱的ITS1和ITS2侧翼区(18S,5.8S和28S)序列相对稳定,但ITS1和ITS2序列在3种飞虱中变异较大。ITS1在所分析的438个位点中可变位点达294个,ITS2在分析的403个位点中可变位点为177个。根据3种飞虱rDNA的ITS1和ITS2序列设计了特异性引物,应用特异性引物对样品进行了PCR扩增,分析发现3种飞虱ITS1区的特异性引物扩增效果不理想,而ITS2区的特异性引物可以稳定地扩增出明显的目的DNA条带。因此,采用ITS2区的特异性引物可以对3种飞虱进行快速的分子鉴定。
译  名：
Molecular identification of Nilaparvata lugens (Stl),Sogatella furcifera (Horvath) and Laodelphax striatellus (Fallén)(Homoptera:Delphacidae) based on rDNA ITS1 and ITS2 sequences
作  者：
LIU Yu-Di,LIN Ke-Jian,HAN Lan-Zhi,HOU Mao-Lin(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
关键词：
Nilaparvata lugens;Sogatella furcifera;Laodelphax striatellus;rDNA;ITS1;ITS2;diagnostic primers;molecular identification
摘  要：
The complete sequences of rDNA ITS1 and ITS2 were determined for the brown planthopper Nilaparvata lugens,the white-backed planthopper Sogatella furcifera and the small brown planthopper Laodelphax striatellus in order to explore the molecular identification method for them.The flanking regions of rDNA-ITS1 and ITS2 of the three planthoppers showed only limited variation,but the sequences of rDNA-ITS1 and ITS2 differed significantly.There are 294 variable sites in the 438 analyzed sites for the ITS1 region,and 177 variable sites in the 403 analyzed sites for the ITS2 region.Species-specific primers of N.lugens,S.furcifera,and L.striatellus were designed based on their rDNA-ITS1 and ITS2 sequences.The results of PCR amplification of rDNA-ITS1 in the three species indicated that the species-specific primers were not applicable.However,the species-specific primers based on the rDNA-ITS2 sequences proved to be useful diagnostic primers for the three planthoppers.It is so concluded that molecular identification of N.lugens,S.furcifera and L.striatellus using the species-specific primers from the rDNA-ITS2 region is feasible.

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