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当前位置: 首页 > 文章 > 土壤脱氢酶与蛋白酶对酞酸酯污染的动态响应 江苏农业学报 2012,28 (3) 87-93
Position: Home > Articles > Dynamic responses of soil dehydrogenase and protease to pollution of PAEs in soil Jiangsu Journal of Agricultural Sciences 2012,28 (3) 87-93

土壤脱氢酶与蛋白酶对酞酸酯污染的动态响应

作  者：
高军;宗春琴;周夏曦;张裕;于小彬
单  位：
淮阴工学院生命科学与化学工程学院
关键词：
酞酸酯;土壤脱氢酶;土壤蛋白酶;主成分分析
摘  要：
为评价农田土壤酞酸酯(Phthalate acid esters,PAEs)污染的生态风险,采用室内模拟法,在土壤受邻苯二甲酸二(乙基-己基)酯[Dis(2-ehylhexyl)phthalate ester,DEHP]和邻苯二甲酸二丁酯(Di-n-butyl phthalate ester,DBP)2种酞酸酯类化合物单一和复合不同污染水平下,测定土壤脱氢酶与蛋白酶活性在培养期内对这2种化合物单一污染与复合污染的动态变化。结果表明:土壤中浓度小于10 mg/kg的DBP和浓度小于20 mg/kg的DEHP对土壤脱氢酶与蛋白酶活性没有明显影响,浓度为20 mg/kg时DBP对土壤脱氢酶表现出抑制-激活-恢复效应;在DBP浓度为20 mg/kg时土壤蛋白酶短期内有明显的激活效应;当土壤中DBP或DEHP污染浓度大于或等于50mg/kg时,土壤蛋白酶与土壤脱氢酶均被显著抑制;DBP与DEHP复合污染对土壤脱氢酶表现出协同抑制效应,而对蛋白酶没有类似的效应特征,主成分分析(PAC)也佐证了以上结果。因此,土壤脱氢酶与蛋白酶可以作为土壤酞酸酯污染生态风险评价的生物学指标,但对酞酸酯不同污染水平,这两种土壤酶活性的响应特征有所差异。
译  名：
Dynamic responses of soil dehydrogenase and protease to pollution of PAEs in soil
作  者：
GAO Jun1,2,ZONG Chun-qin1,ZHOU Xia-xi1,ZHANG Yu1,YU Xiao-bin1(1.College of Life Science and Chemical Engineering,Huaiyin Institute of Technology,Huai'an 223003,China;2.Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake,Jiangsu Province,Huai'an 223300,China)
关键词：
phthalate acid esters;soil dehydrogenase;soil protease;principal component analysis
摘  要：
To assess the ecological risk of phthalate acid esters(PAEs) pollution in farmland,surface soil was collected and incubated in lab after artificially contaminated with DBP and DEHP.The dynamic responses of dehydrogenase and protease activities to different levels of DBP or/and DEHP in soil were studied.There was insignificant effect on either dehydrogenase or protease of no more than 10 mg/kg DBP or 20 mg/kg DEHP in soils within incubation period.However,soil dehydrogenase activity changed with incubation time,being inhibited-activated-reversed in soil contaminated with 20 mg/kg DBP.Soil protease activity was significantly activated in soil with 20 mg/kg DBP in early phase of incubation,and soon afterwards,it was reversed.The activities of dehydrogenase and protease were both inhibited significantly with no less than 50 mg/kg DBP or DEHP in soils.Meantime,a synergistic inhibition effect on dehydrogenase activity was observed in soil mixedly contaminated with DBP and DEHP.However,no equivalent effect was observed on protease activity.These differences were verified by principal component analysis(PCA).Soil dehydrogenase and soil protease are capable of acting as biological indicators for accessing ecological risk of soil contaminated with PAEs.On the other hand,some differences were present between dynamic responses of dehydrogenase and protease to the contamination levels of DBP and/or DEHP in soils.

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