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Position: Home > Articles > Isolation of Clostridium perfringens from sheep and detection of its major toxin genes in part of Xinjiang Chinese Veterinary Science 2012 (8) 796-801

新疆部分地区羊源性产气荚膜梭菌的分离及其主要毒力基因的检测

作  者:
吴建勇;李建军;李娜;段新华;杨学云;王登峰;王治才
单  位:
新疆畜牧科学院兽医研究所
关键词:
产气荚膜梭菌;毒素型;多重PCR;β2毒素;肠毒素
摘  要:
为了解新疆部分地区羊源性产气荚膜梭菌(Clostridium perfringens)的毒型分布,对疑似病例所采病料进行了菌株分离,用多重PCR方法对菌株进行了毒素分型,用PCR方法对β2毒素基因(cpb2)和肠毒素基因(cpe)进行了检测。结果,在分离的39株菌中,毒素A型占66.67%(26/39),毒素B型占2.56%(1/39),毒素D型占30.77%(12/39);66.67%(26/39)的菌cpb2基因阳性,A、D型菌cpb2基因阳性率分别为42.31%(11/26)和100%(12/12);cpe阳性率为51.28%(20/39),其中A型菌cpe阳性率为50.00%(13/26),D型菌cpe阳性率为58.33%(7/12);cpb2和cpe双阳性菌株占38.46%(15/39)。上述结果为防治新疆羊源性产气荚膜梭菌感染提供了基础资料。
译  名:
Isolation of Clostridium perfringens from sheep and detection of its major toxin genes in part of Xinjiang
作  者:
WU Jian-yong,LI Jian-jun,LI Na,DUAN Xin-hua,YANG Xue-yun,WANG Deng-feng,WANG Zhi-cai(Institute of Veterinary Medicine,Xinjiang Academy of Animal Sciences,Urumqi 830000,China)
关键词:
Clostridium perfringens;toxin types;multi-PCR;beta2 toxin;enterotoxin
摘  要:
To understand the prevalent toxin types of Clostridium perfringens in disease sheep in part of Xinjiang,strains were isolated from samples collected from suspected cases.The A,B,C,D toxin types were determined by multi-PCR,and their beta2 toxin gene and enterotoxin gene were cloned by PCR.In result,the toxin types of A,B and D accounted for 66.67%(26/39),2.56%(1/39)and 30.77%(12/39)in 39 isolates,respectively.Additionally,the positive rate of cpb2 gene was 66.67%(26/39),in which type A strains accounted for 42.31%,and type D strains accounted for 100%.The positive rate of cpe gene was 51.28%(20/39),in which type A strains accounted for 50%,and type D accounted for 58.33%.The positive rate of both cpb2 and cpe genes was 38.46%(15/39).The analysis provided information for prevention and control of C.perfringens.

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