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当前位置: 首页 > 文章 > 山东花生茎腐病病原菌研究 中国油料作物学报 2014,36 (4) 524-528
Position: Home > Articles > Research on pathogen of peanut stem rot in Shandong Province Chinese Journal of Oil Crop Sciences 2014,36 (4) 524-528

山东花生茎腐病病原菌研究

作  者：
郭洪参;张悦丽;齐军山;张博;李林;徐作珽;李长松;张广民
单  位：
山东省农业科学院植物保护研究所/山东省植物病毒重点实验室;山东农业大学植物保护学院
关键词：
花生茎腐病;核糖体DNA-ITS序列;病原鉴定
摘  要：
从山东省多地采集的花生茎腐病株上分离到15个茎腐病菌株,选取6个典型菌株,对病原菌进行形态特征、致病性、生物学特性研究。核糖体DNA-ITS序列分析表明,6个菌株的核糖体DNA-ITS序列同源性高达99%。根据病原菌的形态特征、核糖体DNA-ITS序列同源性比较,确认供试6个菌株为同一种病原菌,与GenBank中可可毛色二孢属(Lasiodiplodia)(Diplodia的同物异名)的同源性高达99%以上。根据6个菌株的形态特征、致病性测定结果及生物学特性,并结合核糖体DNA-ITS序列分析,将山东花生茎腐病的病原鉴定为棉色二孢菌(Diplodia gossypina)。
译  名：
Research on pathogen of peanut stem rot in Shandong Province
作  者：
GUO Hong-can;ZHANG Yue-li;QI Jun-shan;ZHANG Bo;LI Lin;XU Zuo-ting;LI Chang-song;ZHANG Guang-min;College of Plant Protection,Shandong Agricultural University;Institute of Plant Protection,Shandong Academy of Agricultural Sciences /Shandong Key Laboratory of Plant Virology;
关键词：
Peanut stem rot;;Ribosomal DNA-ITS;;Pathogen identification
摘  要：
Six representative strains were selected from peanut stem rot plants collected from Shandong Province. Morphological characteristics,pathogenicity and biological characteristics of 6 representative strains were studied. Ribosomal DNA-ITS sequence analysis showed that the sequence homology of these 6 strains was up to99%. The tested 6 isolates were confirmed as one species. The homology of DNA-ITS sequences of the 6 strains was up to more than 99% compared with Lasiodiplodia spp( synonyms Diplodia) in GenBank. According to morphological characteristics,pathogenicity test and biological characteristics of the 6 tested strains,and combined with the ribosomal rDNA-ITS sequence analysis,the pathogen of peanut stem rot from Shandong Province was identified as Diplodia gossypina.

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