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当前位置: 首页 > 文章 > 葡聚糖凝胶Sephadex LH-20柱层析分离纯化蓝莓花色苷的研究 食品工业科技 2016,37 (9) 58-63
Position: Home > Articles > Study on isolation and purification optimization of blueberry anthocyanins by Sephadex LH-20 Science and Technology of Food Industry 2016,37 (9) 58-63

葡聚糖凝胶Sephadex LH-20柱层析分离纯化蓝莓花色苷的研究

作  者：
申芮萌;杨岚;于宁;朱月;朱宁;张海平;张鑫;孙爱东
单  位：
北京林业大学林业食品加工与安全北京市重点实验室;北京林业大学生物科学与技术学院食品科学与工程系
关键词：
蓝莓花色苷;Sephadex LH-20;HPLC
摘  要：
本论文初步探讨了采用Sephadex LH-20凝胶柱层析分离纯化蓝莓花色苷的工艺条件,比较了花色苷在不同缓冲液浓度、不同流速和不同上样量条件下的分离效果,并将最佳分离条件下得到的组分进行紫外可见波长扫描,对其中的花色苷组分进行HPLC检测。结果表明,当以30%的酸化甲醇为洗脱液,采用混合流速进行洗脱,进样量为20 mg时,蓝莓花色苷的分离效果最好,共收集到七个峰组分。经紫外-可见光谱扫描,可确定组分Ⅰ、Ⅱ、Ⅲ为非花色苷类物质;组分Ⅳ、Ⅴ、Ⅵ、Ⅶ为花色苷类物质。由HPLC检测可知:组分Ⅳ中只含一种蓝莓花色苷;组分Ⅵ、Ⅶ均以一种花色苷为主,且其峰面积比例达到85%以上;组分Ⅴ中主要含两种花色苷。
译  名：
Study on isolation and purification optimization of blueberry anthocyanins by Sephadex LH-20
作  者：
SHEN Rui-meng;YANG Lan;YU Ning;ZHU Yue;ZHU Ning;ZHANG Hai-ping;ZHANG Xin;SUN Ai-dong;Department of Food Science,College of Biological Sciences and Biotechnology,Beijing Forestry University;Beijing Foresty University,Key Laboratory of Forestry Food Processing and Security;
关键词：
blueberry anthocyanins;;Sephadex LH-20;;HPLC
摘  要：
The extraction and purification of blueberry anthocyanins by Sephadex LH- 20 were preliminarily disscussed.The respective separating effects of different buffer concentration,flow velocity and sample loading volume were compared and the optimization of the purification condition were selected. The sample components which separated by the optimal condition were scanned on UV- Visible wavelength and identified by HPLC analysis.The results showed that optimal extraction of blueberry anthocyanins conditions were as following : eluant was 30% ethanol,eluting method was mixture velocity elution,sample loading volume was 20 mg,and seven segments can be collected. Based on the scanning of UV- Visible wavelength,segment Ⅰ,Ⅱ,Ⅲ were not anthocyains and segment Ⅳ,Ⅴ,Ⅵ,Ⅶ were anthocyains.HPLC analysis results showed that segment Ⅳcontained one anthocyanin,segment Ⅵ,Ⅶ mainly contained one anthocyanin respectively and the peak area ratio reached over 85%,segment Ⅴ mainly contained two anthocyanins.

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