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当前位置: 首页 > 文章 > 纳米免疫磁珠富集猪肉中的盐酸克伦特罗 食品科学 2013,34 (14) 182-186
Position: Home > Articles > Enrichment of Clenbuterol in Pork Using Immunomagnetic Nanobeads FOOD SCIENCE 2013,34 (14) 182-186

纳米免疫磁珠富集猪肉中的盐酸克伦特罗

作  者：
李超辉;熊勇华;郭亮;吴科盛;梁语嫣
单  位：
南昌大学食品科学与技术国家重点实验室;南昌大学中德联合研究院
关键词：
克伦特罗;富集;纳米磁珠;猪肉
摘  要：
以生物素-链霉亲和素系统为介导,将克伦特罗单克隆抗体与纳米磁珠偶联制备免疫磁珠,建立特异性快速富集猪肉中盐酸克伦特罗的方法。将1mg偶联有23.9μg链霉亲和素(SA)的磁珠与220μg生物素化抗体反应,得到抗体偶联量为77.2μg/mg的免疫磁珠。免疫磁珠最佳吸附、洗脱条件为4mL 0.15mol/L盐酸在剧烈振荡条件下提取1g猪肉匀浆样品中的克伦特罗,提取液中加入0.25mg免疫磁珠在37℃,200r/min振荡吸附5～10min,100μL甲醇洗脱磁珠5min,洗脱液用PB稀释10倍后ELISA方法检测。加标实验结果表明,加标量为0.25～2ng/g时回收率大于80%,相对标准偏差为11.9%～15.7%;加标量为5ng/g时回收率降至61.2%,相对标准偏差为7.8%。
译  名：
Enrichment of Clenbuterol in Pork Using Immunomagnetic Nanobeads
作  者：
LI Chao-hui1,2,XIONG Yong-hua1,2,GUO Liang1,2,*,WU Ke-sheng2,LIANG Yu-yan2(1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China;2.Sino-German Joint Research Insititute,Nanchang University,Nanchang 330047,China)
关键词：
clenbuterol;enrichment;nanomagnetic bead;pork
摘  要：
An immunoextraction method based on anti-clenbuterol(CLE) immunomagnetic nanobeads was developed for the rapid and specific enrichment of CLE in pork.Immunomagnetic nanobeads were prepared by coupling 77.2 μg of biotinylated antibodies onto 1 mg of nano-magnetic beads that were coated with 23.9 μg of streptavidin.The optimal adsorption and desorption conditions were 1 g of homogenized pork extracted with 4 mL of 0.15 mol/L HCl solution with vigorous shaking.Then,0.25 mg of magnetic nanobeads were added to the extract and incubated 37 ℃ for 10 min on a shaker(200 r/min).Totally 100 μL of methanol was mixed with the immunomagnetic nanobeads for 5 min to desorb CLE.The eluate was diluted 10 times with PBS for ELISA determination.The performance of immunomagnetic nanobeads was evaluated by using CLE-spiked samples.The results indicated that the recovery rate of CLE at spiked levels between 0.25 ng/g and 2 ng/g were more than 80% with relative standard deviation of 11.9% to 15.7%.However,the recovery rate of CLE was decreased to 61.2% with relative standard deviation of 7.8% while the spiked concentration was increased to 5 ng/g.

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