中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 2株乳酸菌对鼠结肠癌模型的抑制效果 吉林农业大学学报 2015,37 (5) 596-599+604
Position: Home > Articles > Antitumor Effect of Two Lactobacillus Strains on Mouse Colon Cancer Model Journal of Jilin Agricultural University 2015,37 (5) 596-599+604

2株乳酸菌对鼠结肠癌模型的抑制效果

作  者：
胡静涛;李俊峰;黄海斌;王丹;叶丽萍
单  位：
吉林农业大学校医院;吉林农业大学动物科学技术学院
关键词：
乳酸菌;鼠;结肠癌;抑瘤率;模型
摘  要：
为比较酸奶中分离的短乳杆菌和鼠李糖乳杆菌对消化道肿瘤的潜在抑制效果,在肿瘤模型建立前2周给小鼠分别灌胃2株乳酸菌(2×108cfu),此后建立鼠结肠癌模型以比较2株乳酸菌对其是否具有抑制作用。通过给小鼠皮下注射CT26.WT细胞获得皮下增生物,经病理组织学检测为肿瘤后,通过腹部手术移植皮下瘤块至肠道表面,进而建立鼠结肠癌肿瘤模型。模型建立后通过腹部B超扫描监测肿瘤体积,并于模型建立2周后处死小鼠、分离肿瘤称重,计算抑瘤率。结果表明:皮下注射CT26.WT可以诱导获得皮下肿瘤,腹部手术、OB胶黏附瘤块建立了鼠结肠癌皮下移植瘤模型,荷瘤率为100%,术后存活率为96%以上。模型建立7 d后,腹部B超扫描检测结果表明,灌胃短乳杆菌组荷瘤鼠肿瘤的体积显著低于PBS组(P<0.05),而鼠李糖乳杆菌灌胃组荷瘤鼠肿瘤体积也有下降趋势,但与PBS组相比差异不显著。分离荷瘤鼠肿瘤组织,灌胃2株乳酸菌组小鼠肿瘤重均有所下降,且短乳杆菌组更为明显,短乳杆菌抑瘤率为36.47%,鼠李糖乳杆菌抑瘤率为21.68%。构建了鼠结肠癌原位移植瘤模型,且短乳杆菌对鼠结肠癌具有较好的抑制效果,优于鼠李糖乳杆菌。
译  名：
Antitumor Effect of Two Lactobacillus Strains on Mouse Colon Cancer Model
作  者：
HU Jingtao;LI Junfeng;HUANG Haibin;WANG Dan;YE Liping;College of Animal Science and Technology,Jilin Agricultural University;School Hospital,Jilin Agricultural University;
关键词：
Lactobacillus;;mice;;colon cancer;;inhibition ratio;;model
摘  要：
To compare antitumor effect of Lactobacillus brevis and Lactobacillus rhamnosus strains on gastrointestinal tumor,colon cancer model was constructed after the mice were drenched by these two strains for continuously two weeks as 2 × 108 cfu. CT26. WT cells( cell viability > 95%) were slowly injected subcutaneously into the mice to obtain xenograft. Once the subcutaneous xenograft was determined as tumor tissue by histopathology,one intact tumor block was implanted onto the serosa of the cecum through abdominal operation in order to construct mouse colon caner model.Then,tumor volume was determined by abdominal B ultrasound and the tumor was weighted to count tumor inhibitory. The result showed that colon cancer model was constructed and tumor bearing rate was up to 100%,and postoperative survival rate was up to 96%. After 7 d of post-implantation,asignificant decrease in tumor volume was observed in Lactobacillus brevis group compared to PBS group( P < 0. 05). The tumor size in Lactobacillus rhamnosus group was reduced without significance. Tumor weight in Lactobacillus brevis group after two weeks was reduced by 36. 47% compared to PBS group,which in Lactobacillus rhamnosus was down-regulated by 21. 68%. In conclusion,mouse colon caner model was constructed and Lactobacillus brevis displayed better antitumor effect than Lactobacillus rhamnosus.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46