中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 疫霉31kDa激发子的分离纯化及其诱导烟草细胞死亡机理的初步研究 植物病理学报 2007,37 (3) 278-283
Position: Home > Articles > Purification of a 31 kDa elicitor produced by Phytophthora and its mechanisms of inducing tobacco cell death Acta Phytopathologica Sinica 2007,37 (3) 278-283

疫霉31kDa激发子的分离纯化及其诱导烟草细胞死亡机理的初步研究

作  者：
詹海燕;薛惠明;潘娟;梁五生
单  位：
浙江大学生物技术研究所
关键词：
疫霉;激发子;过敏反应;烟草;ATP合成
摘  要：
通过沸水浴、硫酸铵沉淀、非变性电泳、割胶电洗脱等方法,从一种疫霉的培养液中分离得到分子量约31kDa的蛋白类激发子。该激发子在低浓度下即可诱发烟草叶片发生过敏反应,处理烟草悬浮细胞12h能导致24.6%的细胞死亡,处理24h后细胞的死亡率达67.8%。用该激发子处理烟草悬浮细胞24h后细胞基因组受到明显降解,但无DNAlad-dering现象。用该激发子处理1h即可显著降低烟草悬浮细胞中的ATP水平,而用氧化磷酸化的解偶联剂CCCP处理在降低胞内ATP水平的同时也能显著诱导烟草悬浮细胞死亡,因此抑制胞内ATP合成可能是该激发子诱导烟草细胞死亡的重要机制之一。
译  名：
Purification of a 31 kDa elicitor produced by Phytophthora and its mechanisms of inducing tobacco cell death
作  者：
ZHAN Hai-yan, XUE Hui-ming, PAN Juan, LIANG Wu-sheng (Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China)
关键词：
Phytophthora; elicitor; hypersensitive response; tobacco; ATP synthesis
摘  要：
A proteinaceous elicitor was extracted and purified from the culture filtrate of a Phytophthora isolate through the procedures of boiling-water bath, ammonium sulfate precipitation, non-denaturing polyacry-lamide gel electrophoresis (PAGE), corresponding gel cut and electroelution. It showed an apparent molecular weight of about 31 kDa in SDS-PAGE gel. Inoculation with this elicitor caused necrosis in tobacco leaves. Treating suspension-cultured tobacco cells with the elicitor resulted in 24.6% cell death after 12 h, and 67.8% after 24 h. Obvious genome degradation occurred in suspension-cultured tobacco cells after treatment with the elicitor for 24 h, but no DNA laddering was observed. A significant decrease of ATP level was found in suspension-cultured tobacco cells after treatment with the elicitor for 1 h. As treating the suspension-cultured tobacco cells with carbonyl cyanide 3-chlorophenylhydrazone (CCCP), an oxidative phosphorylation uncoupler, resulted in both obvious intracellular ATP level decrease and cell death, it is inferred that inhibition of ATP synthesis is one of the important mechanisms of inducing tobacco cell death by this elicitor.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46