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Position: Home > Articles > Pathogen isolation,identification and susceptibility test of Ulcerative Disease Syndrome on Pelteobagrus fulvidraco Freshwater Fisheries 2015,45 (5) 100-104

黄颡鱼“溃疡综合征”病原的分离鉴定及药敏试验

作  者:
徐洋;蔺凌云;姚嘉赟;盛鹏程;潘晓艺;尹文林;沈锦玉
单  位:
农业部淡水渔业健康养殖重点实验室浙江省鱼类健康与营养重点实验室浙江省淡水水产研究所
关键词:
黄颡鱼(Pelteobagrus fulvidraco);溃疡综合征;维氏气单胞菌(Aeromonas veronii)
摘  要:
对湖州地区患"溃疡综合征"的黄颡鱼(Pelteobagrus fulvidraco)开展病原研究,从典型濒死的病样体内分离到优势菌TH0426,对该菌进行了人工感染试验,并进行了形态特征、理化特性及分子生物学等方面的综合分析。结果显示,人工感染试验成功复制"溃疡综合征"症状,证明该优势菌为黄颡鱼"溃疡综合征"病原菌,其半数致死量(LD50)为3.98×104CFU/m L。生理生化及16 Sr DNA序列分析结果表明该菌为维氏气单胞菌(Aeromonas veronii)。药敏试验结果显示头孢曲松、利福平、四环素、多粘菌素B、氟苯尼考、头孢噻吩、强力霉素、头孢西丁、诺氟沙星、头孢拉定、氧氟沙星、环丙沙星、氟罗沙星和恩诺沙星等14种药物对该菌有较好的抑制作用。
译  名:
Pathogen isolation,identification and susceptibility test of Ulcerative Disease Syndrome on Pelteobagrus fulvidraco
作  者:
XU Yang;LIN Ling-yun;YAO Jia-yun;SHENG Peng-cheng;PAN Xiao-yi;YIN Wen-lin;SHEN Jin-yu;Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture,Key Laboratory of Fish Health and Nutrition of Zhejiang Province,Zhejiang Institute of Freshwater Fisheries;
关键词:
Pelteobagrus fulvidraco;;Ulcerative Disease Syndrome(UDS);;Aeromonas veronii
摘  要:
A bacterical strain,TH0426,was isolated from diseased Pelteobagrus fulvidraco. An artificial infection test showed that TH0426 was the pathogen of wcerative dasease syndrome on P. fulvidraco and the LD50 was about 3. 98 ×104CFU / m L. Based on its morphological,physiologial and biochemical charateristics and 16 S r DNA molecular analysis,the strain TH0426 was proved to be Aeromonas veronii. The drug sensitivty test indicated that TH0426 was susceptible to 14 kinds of antibiotics,including Ceftriaxone Sodium,rifampicintablets,tetracycline,Polymyxin B sulfate,florfeniol,ceftofur,doxycycline,norfloxacin,cefradex,ofloxacin,ciprofloxacin,cefoxitin,feroxacin and enrofloxacin.

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