Cell-SELEX技术研究进展
作 者：
赵仲麟;李燕;袁超
单 位：
河南农业大学理学院;郑州轻工业学院食品与生物工程学院
关键词：
Cell-SELEX;生物标记物;癌症研究;核酸;适配子
摘 要：
适配子是通过SELEX技术,即指数富集配体系统进化技术,经反复放大筛选得到的、与目标分子有高度亲和性的分子。SELEX技术常使用纯化的靶分子,目前活细胞也可用于筛选目标,这种技术称为细胞指数富集配体系统进化技术(CellSELEX),其优点是产生的适配子可以与活细胞中的靶分子的天然构象发生作用。细胞表面的跨膜蛋白也可作为适配子的目标。此外,在不知道任何细胞表面分子的情况下也可以得到细胞特异性适配子。对Cell-SELEX技术的应用及研究进展进行综述。
译 名：
Research Progress of Cell-SELEX
作 者：
Zhao Zhonglin;Li Yan;Yuan Chao;College of Sciences,Henan Agricultural University;School of Food and Biological Engineering,Zhengzhou University of Light Industry;
关键词：
Cell-SELEX Biomarkers Cancer research Nucleic acids Aptamer
摘 要：
Aptamers have high affinity to target molecules, which are selected by an in vitro process known as cell systematic evolution of ligands by exponential enrichment(Cell-SELEX). SELEX is carried out using purified target molecules, but whole live cells could be used as selection targets in Cell-SELEX progress. The advantage of this technology is that aptamers could be functional with a native conformation of the target molecule on live cells. Cell surface transmembrane proteins would also be the targets. In addition, cell-specific aptamers can be obtained without any knowledge about cell surface molecules on the target cells. In this paper, the progress of Cell-SELEX technology was reviewed.
导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
