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药用植物研究(英文版)
2012,3
(10)
Position: Home > Articles > Tissue culture of the shoot tips of Bozhou Paeonia lactiflora Pall.
Medicinal Plant
2012,3
(10)
Tissue culture of the shoot tips of Bozhou Paeonia lactiflora Pall.
作 者：
MA Zong-xin;Ma TongFu;Meng DongQin
单 位：
Fuyang Teachers College,Fuyang,Anhui,236041,China;Fuyang Teachers College,Fuyang,Anhui,236041,China)
关键词：
shoot tips;bozhou;tissue;culture of;ms;6-b
摘 要：
[Objective] To study the effects of culture conditions on the tissue culture of shoot tips of Bozhou Paeonia lactiflora Pall. [Method] Culture mediums with different concentration combinations of 6-BA, NAA and IBA were prepared. Based on the cultivation results, the optimal formula was selected. [Result] After shoot tips of Bozhou P. lactiflora were cultivated on 1/2 MS+ 1.0mg/L 6-BA for 39d, buds and multiple shoots were differentiated. Multiple shoots showed the rapidest proliferation on 1/2 MS+ 1.0mg/L6-BA, and the optimal rooting results on 1/2 MS+0.1mg/L IBA. [Conclusion] Concentration of plant growth regulator directly affected the tissue culture of shoot tips of Bozhou P. lactiflora. And shoot tips could directly grow into seedlings under appropriate culture medium.
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