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Position: Home > Articles > Comparison of Peptides Expression in Buffalo Follicular Fluid with MALDI-TOF Mass Spectrometry China Animal Husbandry & Veterinary Medicine 2012,39 (1) 132-136

利用MALDI-TOF质谱比较水牛卵泡液多肽变化的研究

作  者:
付强;刘振方;杨丽;张明;卢克焕
单  位:
广西大学动物繁殖研究所亚热带农业生物资源保护与利用国家重点实验室
关键词:
水牛;卵泡液;MALDI-TOF;多肽;特异性表达
摘  要:
为检测卵泡内小肽的表达,采用基质辅助激光解离串联飞行时间质谱(MALDI-TOF/TOF MS)建立了成熟卵泡液的多肽表达模型,比较小卵泡(直径<4mm)、中等卵泡(直径4~8mm)和成熟卵泡(直径>8mm)的多肽表达变化。结果表明,3种形态的卵泡液存在12个显著差异多肽,其中在成熟卵泡内上调6个,下调有5个;在中等卵泡内上调4个,下调5个。其中分子质量约为1746u的多肽在成熟卵泡中特异性表达,可作为卵泡成熟的潜在生物标志物之一。
译  名:
Comparison of Peptides Expression in Buffalo Follicular Fluid with MALDI-TOF Mass Spectrometry
作  者:
FU Qiang,LIU Zhen-fang,YANG Li,ZHANG Ming,LU Ke-huan(State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources;Animal Reproduction Institute,Guangxi University,Nanning 530004,China)
关键词:
buffalo;follicular fluid;MALDI-TOF;peptide;specific expression
摘  要:
In order to detect the peptides expression profile in follicles,the matrix-assisted laser desorption ionization mass spectrometry was used to establish a model of peptide expression profile and compare expression changes of peptides from small follicles((φ<4 mm),medium follicules(4 to 8 mm) and mature follicles(φ>8 mm).The results indicated that there were 12 peptides to express significantly different among three shapes of follicles.6 peptides increased and 5 peptides decreased in mature follicles.The 1746 u of peptide expressed specifically in mature follicles,which might be used as the one of the underlying biomarkers for follicles maturation.

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