当前位置: 首页 > 文章 > 基于iTRAQ技术的黄曲霉胁迫花生蛋白质组分析 花生学报 2020 (1) 25-30+18
Position: Home > Articles > Analysis of Differential Proteome in Peanut under Aspergillus flavus Stress Based on iT RAQ Technique Journal of Peanut Science 2020 (1) 25-30+18

基于iTRAQ技术的黄曲霉胁迫花生蛋白质组分析

作  者:
李春娟;闫彩霞;王娟;孙全喜;苑翠玲;单世华;赵小波
单  位:
山东省花生研究所
关键词:
花生;黄曲霉;蛋白质组;几丁质酶
摘  要:
以花生抗黄曲霉品种J11为研究对象,利用蛋白质组测序技术(iTRAQ)分析了黄曲霉侵染胁迫条件下,花生蛋白质组表达变化情况,共鉴定到1382个蛋白质,其中84个蛋白质的表达发生变化,包括74个上调表达与10个下调表达。综合GO与KEGG分析的结果,发现黄曲霉侵染主要影响花生的代谢通路,且诱导植株抗性机制的运行。此外,本研究从生物信息学角度,对花生Ahhevamine-A基因进行了深入分析。本研究结果可为筛选新的抗黄曲霉花生种质提供理论依据。
译  名:
Analysis of Differential Proteome in Peanut under Aspergillus flavus Stress Based on iT RAQ Technique
作  者:
LI Chun-juan;YAN Cai-xia;WANG Juan;SUN Quan-xi;YUAN Cui-ling;SHAN Shi-hua;ZHAO Xiao-bo;Shandong Peanut Research Institute;
关键词:
Arachis hypogaea;;Aspergillus flavus;;proteome;;chitinase
摘  要:
By using of iTRAQ technique, this study tried to gain expression and regulation pat-terns of key peanut proteins response to Aspergillus flavus. The proteomic profiling showed that 84 of the 1382 detected protein candidates were aberrantly expressed as a result of A. flavus invasion, including 74 up-regulated and 10 down-regulated. Taken GO and KEGG together, these results con-firmed that A. flavus infection could greatly alter the metabolism and induce host defense mecha-nisms in the peanut, echoing a number of previous studies on the broad impact that the invasion of fungal pathogens could exert on global gene expression in plant. This study also deeply analyzed the Ahhevamine-A gene based on bioinformatics. This research provided theoretical basis for the screen-ing of A. flavus resistant germplasms from peanut.

相似文章

计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊