Effects of GPNMB on Melanogenesis in Mouse Melanocytes

ZHAO Bing-ling;CHEN Tian-zhi;YANG Yu-jing;YU Xiu-ju;YANG Lin-pei;DONG Chang-sheng;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;Shenzhou of Hebei Province Vocational and Technical Education Center;

GPNMB;;UVB;;melanocytes;;melanin

This study aims to investigate whether GPNMB differentially expresses in the skin of mice with various hair colors and to determine its effect on the melanin synthesis.Three approximately 12-day-old healthy C57BL/6mice were randomly selected from strains with black,brown and gray coat colors,respectively.Three pieces of dorsal skin tissue were collected from each mouse using a skin collector after shaving.Immunohistochemistry assay revealed that GPNMB was mainly located in the hair follicle matrix,the inner and outer root sheath,and the dermal papilla in the skin with various hair colors.The Western blot analysis and qRT-PCR assay showed that the expression level of GPNMB was the lowest in the skin of gray mice.Compared with the control group,relative levels of MC1 R,β-catenin and SLC45A2 significantly increased in mouse melanocytes overexpressing GPNMB.we detected the levels of GPNMB and MC1 Rin mouse melanocytes which had been radiated by UVB.The results showed that UVB had an impact on the GPNMB and MC1 Rexpression,and the level of GPNMB was various with different UVB radia-tion doses.The above results suggested that GPNMB was differentially expressed in the skin of mice with different hair colors,and it was involved in the formation of the hair color by regulating the melanin synthesis.