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Position: Home > Articles > Molecular Cloning and Sequence Analysis of Partial 18SrDNA of Mold in Koji FOOD SCIENCE 2009,30 (1) 211-213

甜酒曲中霉菌18SrDNA克隆及其序列分析

作  者:
李再新;雷云玲;刘达玉;谢万如;向超
单  位:
四川理工学院生物工程系
关键词:
18SrDNA;米根霉;甜酒曲
摘  要:
以甜酒曲霉菌为研究靶点,提取霉菌基因组DNA,利用PCR技术扩增该霉菌18SrDNA部分基因序列,构建pKRX-T筛选载体,转化E.coil HB101。经测序和相似性分析,结果发现该霉菌菌株与米根霉(Rhizopus oryzae)有99%同源性,推测该菌可能为米根霉的突变株。
译  名:
Molecular Cloning and Sequence Analysis of Partial 18SrDNA of Mold in Koji
作  者:
LI Zai-xin~(1,2) LEI Yun-ling~1 LIU Da-yu~1 XIE Wan-ru~1 XIANG Chao~1 1.Department of Biotechnology Engineering,Sichuan University of Science and Engineering,Zigong 643000,China; 2.College of Bioengineering,Chongqing University,Chongqing 400044,China
关键词:
18SrDNA;;Rhizopus oryza;;koji
摘  要:
Genome DNA extracted from mold in koji was acted as model in the partial 18SrDNA sequence-amplified reaction by PCR.The DNA sequences were inserted into screening vector pKRX-T,and then were transformed into E.coil HB101.The results of sepuencing and simlarity analysis showed that the mold strain is similar to Rhizopus oryzae at 99 %.This strain might be regarded as mutant ofRhizopus oryzae.

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