当前位置: 首页 > 文章 > 生石花种子萌发及幼苗生长最优条件的筛选 安徽农业科学 2016,44 (18) 123-126
Position: Home > Articles > Screening of Different Mediums and Hormone Concentrations for Seed Germination and Growth Conditions of Lithops Journal of Anhui Agricultural Sciences 2016,44 (18) 123-126

生石花种子萌发及幼苗生长最优条件的筛选

作  者:
范丽楠;张宗申;刘平武
单  位:
广西大学农学院;大连工业大学生物工程学院
关键词:
生石花;培养基;种子萌发;激素;光照时间
摘  要:
[目的]优化生石花种子萌及幼苗生长的条件,以提高其种子萌发率。[方法]采用MS、1/2MS、3/4MS等培养基为生石花生长基质,比较不同培养基中种子萌发率和幼苗生长情况,在此基础上进行光照时间、激素配比和浸泡时间的筛选。[结果]1/2MS培养基最适合生石花种子萌发及幼苗生长。16 h为最优光照时间;不同因素影响由大到小依次为浸泡时间、GA浓度、IAA浓度、NAA浓度及6-BA浓度。其中,GA浓度与浸泡时间对种子萌发有显著影响,且0.1 mg/m L的GA浓度以及4 h的浸泡时间为最优组合。[结论]该研究为生石花组织培养快繁技术研究奠定基础。
译  名:
Screening of Different Mediums and Hormone Concentrations for Seed Germination and Growth Conditions of Lithops
作  者:
FAN Li-nan;ZHANG Zong-shen;LIU Ping-wu;School of Aonomy,Guangxi University,State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources;School of Biological Engineering,Dalian Polytechnic University;
关键词:
Lithops;;Culture medium;;Seed germination;;Hormone;;Illumination time
摘  要:
[Objective] To optimize the seed germination condition of Lithops,and to enhance the germination rate of seeds using MS culture medium to replace soil as growth medium of Lithops. [Method] Compared with the seed germination rate,root growth and seedling growth in different culture mediums,we screened the illumination time,hormone proportion and soaking time. [Result]1 /2 MS culture medium is the most suitable culture medium for the germination and growth of Lithops seeds. Illumination for 16 h was the optimal time. The factors influencing degree from big to small was soaking time,GA concentration,IAA concentration and 6-BA concentration. Among them,GA concentration and soaking time had significant effects on seed germination. And 0. 1 mg / m L GA and 4 h soaking time were the optimal combination.[Conclusion]This research provides basis for the rapid propagation technique of Lithops tissue culture.

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