聚酰胺固相萃取法检测动物源食品中全氟辛酸和全氟辛烷磺酸
作 者：
林钦
单 位：
福建省产品质量检验研究院
关键词：
超高效液相色谱-串联四极杆质谱仪;动物源食品;全氟辛酸;全氟辛烷磺酸;聚酰胺固相萃取
摘 要：
建立动物源食品中全氟辛酸(PFOA)和全氟辛烷磺酸(PFOS)的聚酰胺固相萃取法.样品采用酸化乙腈提取,在酸性条件下用聚酰胺固相萃取小柱富集、净化,5%氨水-甲醇溶液洗脱后采用超高效液相色谱-串联四极杆质谱检测.PFOA和PFOS在1~60ng/mL质量浓度范围内均呈良好的线性关系,r=0.9999.精密度在0.8%~6.2%之间(n=5);两种待测组分的检测限均为0.2μg/kg;对6种动物源食品的加标回收率在93.3%~102.8%之间.
单 位：
Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002,China
关键词：
ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)%animal-origin food%perfluorooctanoic acid(PFOA)%perfluorooctane sulphonic acid(PFOS)%polyamide solid-phase extraction
摘 要：
A method for the extraction of perfluorooctanoic acid(PFOA) and perfluorooctane sulphonic acid(PFOS) in foods of animal origin using polyamide solid-phase extraction(SPE) was developed.Samples were extracted with acidified acetonitrile,cleaned up and enriched on a polyamide SPE cartridge under acidic conditions through elution with 5% ammonia-methanol and determined by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/ MS).The calibration curves for PFOA and PEOS exhibited good linearity(r = 0.9999) in the concentration range of 1 to 60 ng/mL,and the detection limit of the developed method was 0.2 μg/kg for both compounds and the average recovery rates were in the range of 93.3% to 102.8% with relative standard deviations(RSD) of 0.8% to 6.2%.
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