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Freshwater Fisheries
2014
(1)
43-47
转基因斑马鱼侧线系统的形成及其对新霉素的抗性
作 者：
邵贝丽;严继舟
单 位：
上海海洋大学水产与生命学院
关键词：
斑马鱼(Danio rerio),侧线系统;神经丘;毛细胞;新霉素
摘 要：
利用转基因斑马鱼(Danio rerio)189b动态观察侧线系统形成及其对新霉素处理的敏感性。试验结果显示,转基因斑马鱼荧光细胞出现、迁移和定居与侧线神经丘的形成走向和发育时间基本吻合。随着发育时间的推移,荧光点的形态也发生了较大变化,由最初的比较模糊的聚集在一起的荧光团,逐渐变成颗颗分明的荧光点,大小也由大变小。将受精后24 h和6 d带有荧光的细胞暴露于150μmol/L的氨基糖甙类抗生素(新霉素)中,并不影响荧光细胞的数量和分布。结果说明荧光标记细胞对新霉素处理不敏感,不是毛细胞,可能是神经丘形成的先祖细胞。
译 名：
Development of lateral line system and its resistance to neomycin in transgenic zebrafish
作 者：
SHAO Bei-li;YAN Ji-zhou;College of Fisheries and Life Sciences,Shanghai Ocean University;
关键词：
Danio rerio;;lateral line;;neuromasts;;hair cells;;neomycin
摘 要：
In this experiment gene- targeting GFP transgenic zebrafish 189 b was used to observe the whole development process of lateral line system and the responses to neomycin treatment. The results showed that the occurrence and distribution of GFP- labeling cells were highly consistent with the spatiotemporal development of the neuromasts along the lateral line,especially in migration route and final residence. As the larvae grew,the diffused migratory GFP- cells focused as small and bright GFP- dots. Exposure of 24 h and 6 d of GFP- positive embryos in 150 μmol / L of amino sugar glucoside class antibiotic( neomycin) did not change the GFP- pattern and distribution. These results suggested that the GFP- labeling cells might be neuromast progenitors and resistant to neomycin.
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