Position: Home > Articles > Study on Increase of Haploid Wheat Plantlet from Wheat × Tripsacum Dactyloide Hybridization
Journal of Shanxi Agricultural Sciences
2008,36
(11)
44-46
提高小麦×鸭茅状摩擦禾杂交组合的小麦单倍体植株成苗率的探索
作 者：
刘少翔;闫贵云;孙玉;孙善澄
单 位：
山西省农业科学院作物遗传研究所
关键词：
小麦×鸭茅状摩擦禾;幼胚培养;单倍体植株
摘 要：
法国小麦和中国小麦杂交的后代和鸭茅状摩擦禾(父本)杂交,授粉1 d后用100 mg/L 2,4-D蘸穗处理,12~14 d取单倍体幼胚培养。结果表明,仅培养于1/2 MS培养基上,成苗率为78.01%;而根据幼胚的发育状态,先将发育较完善的幼胚直接接种在1/2 MS培养基上,将发育不良的幼胚先接种于1/2 MS+2 mg/L 2,4-D的培养基上进一步养育幼胚,3~4 d后再将其转入1/2 MS培养基中,此法的成苗率为93.22%,较前者的成苗率提高了15.21个百分点。
译 名：
Study on Increase of Haploid Wheat Plantlet from Wheat × Tripsacum Dactyloide Hybridization
作 者：
LIU Shao-xiang,YAN Gui-yun,SUN Yu,SUN Shan-cheng(Institute of Crop Genetics,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
关键词：
Wheat×Tripsacum dactyloides;Immature embryo culture;Haploid plantlet
摘 要：
Generations of F1,F2 and F3 of combinations of Chinese wheat with French wheat as female parents crossed with Tripsacum dactyloides.Wheat ears were dipped in solution contained 100 mg/L 2,4-D pollinated in 24 hours.Immature haploid embryos were cultured in medium of 1/2 MS or 1/2 MS+2 mg/L 2,4-D after pollinated 12～14 days.Plant percent in 1/2 MS medium is 78.01%.In fact,there exists a great difference in these embryos and the plant percentage could reach 93.22% if the haploid embryos were cultured according to the situation of their development.To a incomplete embryo,it could be nurtured in medium with 2,4-D for 3～4 days,then turned to 1/2 MS medium without phytohormone.And to a perfect embryo,it could be cultured in 1/2 MS medium directly,and must be transferred onto the 1/2 MS+2 mg/L 2,4-D if it could not germinate in 3～4 days because the sensation of our eye are not so correct.
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