Shansi Tian;Hao YongBing;Shang XueFan

Department of Chemistry, Xinxiang Medical University, Xinxiang, Henan 453003 Chin;Department of Chemistry, Xinxiang Medical University, Xinxiang, Henan 453003 China

folium nelumbinis;zn;zinc;standard;solution;pa

[Objective]To determine the Zn content in FOLIUM NELUMBINIS,so as to provide scientific basis for the deep development and utilization of FOLIUM NELUMBINIS in pharmaceutical and food industries.[Method]Content of Zn in FOLIUM NELUMBINIS was determined by direct UV spectrophotometry using PAN as the color-developing agent and with standard zinc solution as control at the wavelength of 562 nm.[Result]Zn(Ⅱ) and PAN could form red complex,and the absorbance presented good linear relationship with concentration of standard zinc solution in the range of 0.48-5.03 μg/ml(r=0.999 9).The mean content of Zn in FOLIUM NELUMBINIS was 87.44 μg/g with average recovery of 99.8% and RSD value of 0.34%.[Conclusion]Simple in operation,accurate in determining result and good in repeatability and stability,the spectrophotometry established in this case can be used for determining the content of zinc in FOLIUM NELUMBINIS.