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2018年广西活禽市场新城疫病毒分子流行病学分析

作  者:
曾婷婷;谢芝勋;谢丽基;张艳芳;罗思思;谢志勤;黄娇玲;张民秀;范晴;王盛
单  位:
广西壮族自治区兽医研究所
关键词:
新城疫病毒;活禽市场;分子流行病学调查
摘  要:
为跟踪调查广西活禽市场新城疫病毒(Newcastle disease virus,NDV)的流行分布和分子演化特征,2018年在广西不同城市10个活禽市场随机采集鸡、鸭、鹅和鸽子泄殖腔和咽喉拭子样品2 820份,通过病毒分离与F基因扩增和测序,分析其遗传进化特征。结果显示:从4种宿主中共分离得到54份NDV,包含CLASSⅠ基因1b亚型、CLASSⅡ基因Ⅰ型和CLASSⅡ基因Ⅻ型三种基因型,且发现同一宿主存在不同基因型混合感染的情况。CLASSⅠ基因1b亚型NDV占比最大(50/54,92.5%)。遗传进化分析显示,广西分离株与同基因型参考株处于不同的小分支,存在地域差异。
译  名:
Molecular Epidemiology of Newcastle Disease Viruses from Guangxi Live Poultry Markets in 2018
作  者:
ZENG Tingting;XIE Zhixun;XIE Liji;ZHANG Yanfang;LUO Sisi;XIE Zhiqin;HUANG Jiaoling;ZHANG Minxiu;FAN Qing;WANG Sheng;Guangxi Veterinary Research Institute;
关键词:
Newcastle disease virus(NDV);;live poultry markets;;molecular epidemiological
摘  要:
To study the epidemic distribution and molecular characteristics of Newcastle disease virus(NDV) in Guangxi live poultry markets, 2 820 tracheal or cloacal swab samples of chickens, ducks, geese, pigeons were collected randomly from 10 live poultry markets in Guangxi, 2018. NDV were identified by virus-isolation and F genes were sequenced and analyzed. Fifty-five NDV were isolated. Molecular epidemiological analysis showed that ClassⅡ genotypeⅠ NDV, ClassⅡ genotype Ⅻ NDV and ClassⅠ subgenotype 1 b NDV were included and some of isolates were coinfected by mixed genotypes.Phylogenetic analysis showed that Guangxi isolates were in a independent branch compared with the same genotype references isolates, indicated that some genetic differences existed in different areas.

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