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当前位置: 首页 > 文章 > 禁食条件下草鱼肠粘膜可培养纤维素降解菌的分离与鉴定 淡水渔业 2013,43 (4) 23-28
Position: Home > Articles > Isolation and identification of major cellulase-producing bacteria in intestines of Ctenopharyngodon idellus under fasting conditions Freshwater Fisheries 2013,43 (4) 23-28

禁食条件下草鱼肠粘膜可培养纤维素降解菌的分离与鉴定

作  者：
李欢;钟秋萍;王微微;吴山功;邹红;李文祥;王桂堂
单  位：
中国科学院水生生物研究所
关键词：
草鱼(Ctenopharyngodon idellus);禁食;纤维素降解菌;16 SrDNA。
摘  要：
为探究草鱼(Ctenopharyngodon idellus)肠道固有纤维素降解菌群及其酶活,从冬天禁食条件下的野生草鱼肠道粘膜分离培养的细菌中随机挑取22个单菌落,经筛选得到8株纤维素降解细菌,测量了它们降解纤维素所产生的水解圈的大小,并进行了16 S rDNA序列测定。结果显示,不同细菌(菌株)之间纤维素降解能力存在显著差异(F=4.03,P<0.05),其中GCM4和GCM8的纤维素降解能力较强,D/d值分别为4.37和4.14;GCM1的纤维素降解能力较弱,D/d值为1.99。序列测定结果表明,菌GCM1与碘短杆菌(Brevibacterium iodinum)相似性达99%,GCM2-GCM7与温和气单胞菌(Aeromonas sobria)相似性达到99%,GCM8与Bacillus indicus相似性达99%。
译  名：
Isolation and identification of major cellulase-producing bacteria in intestines of Ctenopharyngodon idellus under fasting conditions
作  者：
LI Huan1,2,ZHONG Qiu-ping1,2,WANG Wei-wei1,2,WU Shan-gong1,ZOU Hong1, LI Wen-xiang1,WANG Gui-tang1,2(1.The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences,Institute of Hydrobiology, Chinese Academy of Sciences,Wuhan 430072,China; 2.University of Chinese Academy of Sciences,Beijing 100039,China)
关键词：
Ctenopharyngodon idellus;fasting;cellulase-producing bacteria;16 S rDNA.
摘  要：
In order to explore Ctenopharyngodon idellus inherent cellulase-producing bacteria and their enzyme activity,randomly picked 22 single strains of bacteria in the intestinal mucosa were isolated and cultured from wild C.idellus in the winter under fasting conditions.Total 8 cellulase-producing strains were screened from the intestinal mucosa of wild C.idellus,and the diameter of hydrolyzing zones and colonies by degradation of cellulose were measured.Cellulose degradation capacity exited a significant difference(F=4.03,P<0.05) between the different bacteria(strain),and strain GCM4 and GCM8 were stronger while GCM1 was the weaker,the D/d values were 4.37,4.14 and 1.99,respectively.16 S rDNA genotype identification indicated that GCM1,GCM2-GCM7,GCM8 were related(99% similarities) to Brevibacterium iodinum,Aeromonas sobria,Bacillus indicus,respectively.

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