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当前位置: 首页 > 文章 > 齐口裂腹鱼生长、肌肉品质和脂蛋白脂酶及脂肪酸合成酶基因表达的研究 食品科学 2015,36 (1) 164-169
Position: Home > Articles > Growth, Muscle Quality and Gene Expression of Lipoprotein Lipase and Fatty Acid Synthase of Schizothorax prenanti Tchang FOOD SCIENCE 2015,36 (1) 164-169

齐口裂腹鱼生长、肌肉品质和脂蛋白脂酶及脂肪酸合成酶基因表达的研究

作  者：
夏晓杰;邬应龙;冯姣;曾丽萍;周成
单  位：
四川农业大学食品学院
关键词：
齐口裂腹鱼;生长;品质;脂蛋白脂酶;脂肪酸合成酶
摘  要：
目的:研究不同生长阶段齐口裂腹鱼的生长状况、肌肉品质和脂蛋白脂酶(lipoprotein lipase,LPL)及脂肪酸合成酶(fatty acid synthetase,FAS)的基因表达。方法:分别选取齐口裂腹鱼幼鱼(137.8±1.7) g(S组)、亚成鱼(312.4±11.8) g(M组)、成鱼(500.9±31.4) g(L组),测定形体指标、脏器指数和肌肉主要营养成分含量,并采用实时荧光定量聚合酶链式反应测定LPL及FAS的基因表达水平。结果:与S组相比,M组、L组肥满度、肝体比、肠脂比、水分含量均有所降低(P<0.05),粗蛋白、粗灰分含量变化不大;L组肌间脂肪(intermuscular fat,IMF)含量最低(P<0.05);而S组、M组与L组肝胰脏中LPL m RNA及FAS m RNA表达水平均基本高于肌肉(P<0.05),且L组肝胰脏中LPL m RNA及FAS m RNA表达水平均最低(P<0.05),M组肌肉中LPL m RNA及FAS m RNA表达水平最高(P<0.05)。结论:齐口裂腹鱼的肌肉主要营养成分含量在成鱼阶段有所降低,且IMF含量与LPL m RNA及FAS m RNA表达水平呈一定正相关。
译  名：
Growth, Muscle Quality and Gene Expression of Lipoprotein Lipase and Fatty Acid Synthase of Schizothorax prenanti Tchang
作  者：
XIA Xiaojie;WU Yinglong;FENG Jiao;ZENG Liping;ZHOU Cheng;College of Food Science, Sichuan Agricultural University;
关键词：
Schizothorax prenanti Tchang;;growth;;quality;;lipoprotein lipase;;fatty acid synthase
摘  要：
Objective: To investigate the growth, muscle quality and gene expression of lipoprotein lipase(LPL) and fatty acid synthase(FAS) of Schizothorax prenanti Tchang(SPT) at different growth stages. Methods: SPT with body weights of(137.8 ± 1.7) g(small, juvenile),(312.4 ± 11.8) g(medium, subadult) and(500.9 ± 31.4) g(large, adult) were collected, respectively. Their body condition indexes, visceral indexes and major muscle nutrients were determined. Meanwhile, LPLand FAS m RNA levels in liver and muscle of SPT at the juvenile, subadult and adult growth periods were evaluated by realtime PCR. Results: Compared with the small group, boby condition indexes, hepatopancreas index(HIS), mesenteric lipid index and water content were reduced in the medium group and large group(P < 0.05). The contents of crude protein and crude ash showed no significant change in muscle. The intermuscular fat was the least abundant in the large group(P < 0.05). The LPL and FAS m RNA expression levels in liver for the small group, medium and large group were significantly higher than in muscle(P < 0.05). The LPL m RNA and FAS m RNA expression levels in liver were the lowest in the large group(P < 0.05). The LPL m RNA and FAS m RNA expression levels in muscle were the highest in the medium group(P < 0.05). Conclusion: The essential nutrients in muscle are reduced at the adult stage, and the content of intramusclar fat is correlated with the expression of LPL m RNA and FAS m RNA.

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