单 位：
东北农业大学农学院;浙江大学农业与生物技术学院/农业农村部作物病虫分子生物学重点开放实验室
关键词：
萤蔺;吡嘧磺隆;抗药性;乙酰乳酸合成酶(ALS);谷胱甘肽—硫—转移酶(GSTs);黑龙江省
摘 要：
【目的】明确黑龙江省部分稻田萤蔺(Scirpus juncoides Roxb.)对吡嘧磺隆的抗药性水平及抗性机制,为水稻田萤蔺的综合治理提供理论依据。【方法】以采自黑龙江省的23个萤蔺种群为试验材料,利用整株盆栽法测定其对吡嘧磺隆的抗药性水平。选取代表性抗性种群(SC-1和SC-8)和敏感种群(SC-23),于萤蔺抽茎期剪取花茎后,以不同浓度(0.1、10.0、100.0、1000.0和10000.0 nmol/L)的吡嘧磺隆溶液对其进行离体乙酰乳酸合成酶(ALS)检测;抽茎期喷施250 g/ha吡嘧磺隆对萤蔺进行茎叶处理,施药后第0、1、3、5、7和9 d剪取花茎,分别用于活体ALS活性和谷胱甘肽—硫—转移酶(GSTs)活性测定。【结果】整株盆栽法测定结果表明,供试22个种群中有7个种群对吡嘧磺隆产生了抗性,占供试种群的31.8%。离体条件下,抗性种群SC-1和SC-8体内ALS对吡嘧磺隆的敏感性明显降低,抗性指数分别为69.81和23.83;活体条件下,抗性种群SC-1和SC-8的ALS相对活性受到抑制后均能恢复至正常水平,敏感种群SC-23则不能恢复,3个种群试验结束时(第9 d)的相对活性别为0.92、0.72和0.45;抗性种群SC-1和SC-8的GSTs相对活性自施用吡嘧磺隆后第1 d起均显著高于敏感种群SC-23(P<0.05),且二者分别于施药后第3和第1 d升至最高值,分别为2.36和1.94,敏感种群SC-23的GSTs相对活性于施药后第1 d升至最高值,为1.49。【结论】黑龙江省部分地区稻田萤蔺已对吡嘧磺隆产生了低到中等水平抗性。抗性萤蔺种群的ALS对吡嘧磺隆敏感性下降和GSTs代谢作用增强可能与其抗性有关。
译 名：
An analysis on the resistance of some Scirpus juncoides Roxb. populations in rice fields from Heilongjiang Province to pyrazosulfuron-ethyl
作 者：
LIU Ya-guang;WEI Chao-yue;ZHANG Zheng-nan;ZHU Jin-wen;WU Hui-peng;College of Agronomy,Northeast Agricultural University;College of Agricultural Science and Biotechnology,Zhejiang University/Key Laboratory of Molecular Biology of Crop and Pest,Ministry of Agriculture and Rural Affairs;
关键词：
Scirpus juncoides Roxb.;;pyrazosulfuron-ethyl;;disease resistance;;acetolactate synthase(ALS);;glutamione-sulfur-transferase(GSTs);;Heilongjiang Province
摘 要：
【Objective】Resistance level and resistance mechanism of some Scirpus juncoides Roxb. populations in rice fields from Heilongjiang Province to pyrazosulfuron-ethyl were studied to provide a theoretical basis for the comprehensive management of S. juncoides Roxb. in paddy field.【Method】Who-plant bioassays was used to detect the resistance of 23 S. juncoides Roxb. populations which were collected from Heilongjiang Province to pyrazosulfuron-ethyl. Representative resistant populations(SC-1,SC-8)and sensitive populations(SC-23)were selected as the test materials,stems were cut at the stooling stage followed by different concentrations of pyrazosulfuron-ethyl(0.1,10.0,100.0,1000.0,10000.0 nmol/L)solution for acetolactate synthetase(ALS)test in vitro. In order to determine the ALS activity in vivo and glutathione-sulfur-transferase(GSTs)activity,foliage treatment was applied with 250 g/ha pyrazosulfuron-ethyl at the stooling stage and the stems were cut at 0,1,3,5,7 and 9 d after the application,respectively.【Result】The who-plant bioassays results showed that there were seven populations out of twenty tested populations which were resistant to pyrazosulfuron-ethyl,accounted for 31.8% of the total tested populations. The sensitivity of ALS to pyrazosulfuron-ethyl were decreased in both resistant populations SC-1 and SC-8 in vitro with resistance indexes of 69.81 and 23.83,respectively.The relative enzymatic activity of ALS in both resistant populations(SC-1 and SC-8)could be recovered to normal after inhibition in vivo,while that of the sensitive population SC-23 could not recover,and at the end of the experiment(day 9),the values were 0.92,0.72 and 0.45,respectively. The relative activities of GSTs in resistant populations(SC-1 and SC-8)were significantly higher than the sensitive population(SC-23)from the first day after application of pyrazosulfuron-ethyl(P<0.05),and they rose to the highest values on day 3 and day 1 after application respectively,which were2.36 and 1.94. The relative activity of GSTs in sensitive population SC-23 rose to the highest value on the first day after application,which was 1.49.【Conclusion】Partial S. juncoides Roxb. populations in rice fields from Heilongjiang Province has established low to medium resistance to pyrazosulfuron-ethyl. Decreased sensitivity of ALS of resistant S. juncoides Roxb. populations to pyrazosulfuron-ethyl and increased GSTs metabolism might be related to the resistance.
